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In order to study the effect of sowing date on seed yield of sesame new lines and varieties in Behbahan region an experiment was conducted in two years (2011–2013) at Behbahan Agricultural Research Station. A split plots experiment in randomized complete blocks design with three replications was used. Sowing date was considered as main factor ( Jun. 25, Jul. 11 and Jul. 26 ). Genotype was considered as sub factor (L5-84215 and L5-86365 lines, Safiabadi and Yellow white varieties, Behbahan local population ). Two years compound variance analysis results showed that the effects of sowing date, variety and interaction effect of sowing date*variety on seed yield and yield components were significant. The most seed yield produced on Jun. 25 and Jul. 26 sowing dates (892.46 and 883.01 kg/ha respectively). Behbahan local population and Yellow white variety produced the heightest seed yield (1166.70 and 951.52 kg/ha respectively). In interaction effect of sowing date*variety Behbahan local population on Jul. 26 and Jun. 25 sowing dates produced the heightest seed yield (1210.65 and 1186.27 kg/ha respectively). Therefore according to obtained results and outbreak of charcoal rot on the end of June and beginning of July sowing dates recommended sesame planted with Behbahan local population and Yellow white variety on the end of July in Behbahan region.