همسانه سازی ژن5 AtCDPK جهت اﻳﺠﺎد ﮔﻴﺎﻫﺎن ﺗﺮارﻳﺨﺖ ﻣﻘﺎوم ﺑﻪ ﺗﻨﺶ ﻫﺎی ﺷﻮری و ﺧﺸﻜﻲ

Enviromental stresses are the the most important factors limiting crops production worldwide. Being a member of CDPK family, AtCDPK5 gene plays an important role in plant defense responses to abiotic stresses including drought and salinity. Genetic engineering of these genes could be used for developing stress resistant varieties. The objective of this study was to clone AtCDPK5 for transfer it to plants in order to increase abiotic stress resistance. Total RNA extracted from Arabidopsis thaliana leaves and converted into cDNA. AtCDPK5 was amplified by PCR with specific primers and transformed to pGEM-T easy vector and cloned in E.coli. The integrity of the construct was verified by colony PCR on the clones grown in LB medium containing Ampycilin. The AtCDPK5 cDNA was digested with BamHI and SacI restriction enzymes and the fragment was inserted into pBI121 vector and then cloned in E.coli. The clones were grown in LB medium containing kanamycin and the integrity of the construct was verified by colony PCR and sequencing.