بررسی تنوع ژنتیکی ارقام گندم نان و دوروم با استفاده از نشانگر های ریزماهواره

This study was carried out in genomic laboratory of Islamic Azad University Khorramabad branch. Genetic diversity of 40 wheat cultivars was assessed using 30 microsatellite primers that all of them were generated scorable bands. Totally 71 alleles (ranged between 2 to 4 alleles per each locus) was distinguished. Polymorphic information content (PIC) for all microsatellite primers was calculated. The highest (0.77) and the lowest (0.13) value of PIC was pertained to Xbarc352 and Xcfd56 Primers, respectively. According to similarity matrix, genetic similarity value ranged from 0.18 to 0.95 with an average of 0.48. The lowest and highest genetic similarity was observed between the Sistan and Arg (Bread wheat, No 27 and 28), Karkheh and Behrang (Durum wheat, No 35 and 38) genotypes respectively. Unweighted pair group method of the arithmetic average (UPGMA), based on Jaccard similarity clustering form a dendrogram with three genotypes group. Clustering somewhat was distinguished durum and bread wheat's. Principle coordinate Analysis (PCA), 2D plot was confirmed the results of cluster analysis. Cophenetic correlation showed that molecular data and cluster was corresponded. It was concluded that SSR marker was suitable for evaluated of genetic diversity in wheat genotypes and this genetic diversity can be used in wheat breeding programs.