بررﺳﻲ ﺳﺎﺧﺘﺎر ﺳﻪ ﺑﻌﺪی آﻧﺰﻳﻢ ﭘﺮوﻟﻴﻦ ﻛﺮﺑﻮﻛﺴﻴﻼت رداﻛﺘﺎز در ﮔﻴﺎﻫﺎن

The evaluation of 3D structure and factors influencing on it can offer better perception from the function of protein. P5CR enzyme catalysis end step of biosynthesis of proline pathway. Proline is a osmolite that modulate osmotic presser in cells under abiotic stress. For this purpose, capture 3D Structure and amino acid sequence for Zea mays, Arabidopsis thaliana, Glycine hispidia and Pisuma sativum from ExPASy and NCBI. with use of bioinformatics tools accomplish evaluation on amino acid sequence and 3D structure, secondary structures, location secondary Structures and type and amount of amino acid in polypeptide chain, location of phosphorylation and ligand binding site. These factors are effective on 3D Structure. The surveys indicate that proline is maximum number of amino acid sequence and tryptophan and tyrosine is in minimum number. Alfa helix constitutes approximately 50% of structure of this enzyme, also this enzyme have two domain that one of them is bind to NAD(P) and other is 6-phosphogloconate. The location of phosphorylation is determined and since this enzyme have same function in these plants, conservation region with 156aa determined for it, these region shows active site of enzyme. These factors are effective on 3D structure and therefore they have an impact on function of enzyme.