ﺑﺮرﺳﻲ ﺗﻐﻴﻴﺮات رﻧﮕﻴﺰهﻫﺎی ﻓﺘﻮﺳﻨﺘﺰی ﮔﻴﺎه ﻟﻮﺑﻴﺎ ﺳﺒﺰ (.Phaseoluse vulgaris L) در ﭘﺎﺳﺦ ﺑﻪ ﺗﻠﻘﻴﺢ ﻗﺎرچ Piriformospora indica و ﻣﺤﻠﻮلﭘﺎﺷﻲ ﭘﺎﻛﻠﻮﺑﻮﺗﺮازول

پذیرفته شده برای پوستر
عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ، ﮔﺮوه زراﻋﺖ، داﻧﺸﮕﺎه ﻋﻠﻮم ﻛﺸﺎورزی و ﻣﻨﺎﺑﻊ ﻃﺒﻴﻌﻲ ﺳﺎری
2داﻧﺸﻴﺎرﮔﺮوه زراﻋﺖ، ﭘﮋوﻫﺸﻜﺪه ژﻧﺘﻴﻚ وزﻳﺴﺖ ﻓﻨﺎوری ﻛﺸﺎورزی ﻃﺒﺮﺳﺘﺎن، داﻧﺸﮕﺎه ﻋﻠﻮم ﻛﺸﺎورزی و ﻣﻨﺎﺑﻊ ﻃﺒﻴﻌﻲ ﺳﺎری
3داﻧﺸﺠﻮی دﻛﺘﺮی زراﻋﺖ، داﻧﺸﮕﺎه ﻛﺸﺎورزی و ﻣﻨﺎﺑﻊ ﻃﺒﻴﻌﻲ راﻣﻴﻦ ﺧﻮزﺳﺘﺎن
4اﺳﺘﺎدﻳﺎر ﮔﺮوه ﮔﻴﺎﻫﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه ﻋﻠﻮم ﻛﺸﺎورزی و ﻣﻨﺎﺑﻊ ﻃﺒﻴﻌﻲ ﺳﺎری
چکیده
ی ﺑﺮرﺳﻲ اﺛﺮ ﻗﺎرچ Piriformospora indica و ﭘﺎﻛﻠﻮﺑﻮﺗﺮازول در اﻟﻘﺎی ﺗﺤﻤﻞ ﺑﻪ ﺗﻨﺶ ﺳﺮﻣﺎ در ﮔﻴﺎه ﻟﻮﺑﻴﺎ ﺳﺒﺰ ) Phaseolus vulgaris
L.(، آزﻣﺎﻳﺸﻲ ﮔﻠﺨﺎﻧﻪای ﺑﻪﺻﻮرت ﻓﺎﻛﺘﻮرﻳﻞ ﺑﺮ ﭘﺎﻳﻪ ﻃﺮح ﻛﺎﻣﻞ ﺗﺼﺎدﻓﻲ در ﺳﻪ ﺗﻜﺮار اﺟﺮا ﺷﺪ. ﺗﻴﻤﺎرﻫﺎی آزﻣﺎﻳﺸﻲ ﺷﺎﻣﻞ ﺳﻪ ﺳﻄﺢ ﺗﻨﺶ ﺳﺮﻣﺎ )ﺷﺎﻫﺪ، ﺳﻪ روز و ﺷﺶ روز ﺗﻨﺶ ﺳﺮﻣﺎی 5 درﺟﻪ ﺳﺎﻧﺘﻲﮔﺮاد(، دو ﺳﻄﺢ ﺗﻠﻘﻴﺢ ﻗﺎرﭼﻲ )ﺑﺪون ﺗﻠﻘﻴﺢ و ﺗﻠﻘﻴﺢ ﻗﺎرچ P.
indica( و ﺳﻪ ﺳﻄﺢ ﭘﺎﻛﻠﻮﺑﻮﺗﺮازول )ﺻﻔﺮ، 40 و 80 ﻣﻴﻠﻲﮔﺮم در ﻟﻴﺘﺮ( ﺑﻮد. ﺗﻠﻘﻴﺢ ﻗﺎرچ در ﻣﺮﺣﻠﻪی ﻛﺎﺷﺖ، ﻣﺤﻠﻮلﭘﺎﺷﻲ ﭘﺎﻛﻠﻮﺑﻮﺗﺮازول در دو ﻣﺮﺣﻠﻪی ﭘﻨﺞ و ﺷﺶ ﻫﻔﺘﻪ ﭘﺲ از ﻛﺎﺷﺖ و ﺗﻨﺶ ﺳﺮﻣﺎ ﻫﺸﺖ ﻫﻔﺘﻪ ﭘﺲ از ﻛﺎﺷﺖ اﻋﻤﺎل ﮔﺮدﻳﺪ. ﻧﺘﺎﻳﺞ ﻧﺸﺎن داد ﻛﻪ اﻋﻤﺎل ﺗﻨﺶ ﺳﺮﻣﺎ ﺑﻪوﻳﮋه ﺗﻨﺶ ﺷﺶ روز ﺳﺒﺐ ﻛﺎﻫﺶ وزن ﺧﺸﻚ ﺑﻮﺗﻪ و ﻫﻤﭽﻨﻴﻦ ﻛﻠﺮوﻓﻴﻞ و ﻛﺎروﺗﻨﻮﺋﻴﺪ ﺑﺮگ ﺷﺪ. ﺑﻪ ﻃﻮر ﻛﻠﻲ در ﺗﻤﺎم ﺳﻄﻮح ﺗﻨﺶ ﺳﺮﻣﺎ، ﺗﻠﻘﻴﺢ ﻗﺎرچ P. indica وزن ﺧﺸﻚ ﺑﻮﺗﻪ را اﻓﺰاﻳﺶ داد ﻛﻪ ﺑﻴﺸﺘﺮﻳﻦ اﻓﺰاﻳﺶ در ﺗﻨﺶ ﺳﺮﻣﺎی ﺷﺶ روزه و 65 درﺻﺪ ﺑﻮد. ﻣﺤﻠﻮلﭘﺎﺷﻲ ﭘﺎﻛﻠﻮﺑﻮﺗﺮازول ﺑﻪوﻳﮋه ﻏﻠﻈﺖ 40 ﻣﻴﻠﻲﮔﺮم در ﻟﻴﺘﺮ ﻛﻠﺮوﻓﻴﻞ a و ﻛﺎروﺗﻨﻮﺋﻴﺪ را ﺑﻪ ﺗﺮﺗﻴﺐ 5 و 9 درﺻﺪ اﻓﺰاﻳﺶ داد. درﺣﺎﻟﻲ ﻛﻪ در ﮔﻴﺎﻫﺎن ﻫﻤﺰﻳﺴﺖ ﺷﺪه ﺑﺎ ﻗﺎرچ، ﻣﺤﻠﻮلﭘﺎﺷﻲ ﭘﺎﻛﻠﻮﺑﻮﺗﺮازول وزن ﺧﺸﻚ ﺑﻮﺗﻪ را ﻛﺎﻫﺶ داد ﻛﻪ اﻳﻦ ﻛﺎﻫﺶ در ﻏﻠﻈﺖ 40 ﻣﻴﻠﻲﮔﺮم در ﻟﻴﺘﺮ ﭘﺎﻛﻠﻮﺑﻮﺗﺮازول ﺑﻪ ﺣﺪود 17 درﺻﺪ رﺳﻴﺪ. ﺑﻪ ﻃﻮر ﻛﻠﻲ ﻧﺘﺎﻳﺞ ﻧﺸﺎندﻫﻨﺪه ﻧﻘﺶ ﻣﺜﺒﺖ ﻗﺎرچ P. indica و ﭘﺎﻛﻠﻮﺑﻮﺗﺮازول ﺑﺮ ﺑﻬﺒﻮد رﺷﺪ و اﻓﺰاﻳﺶ ﺗﺤﻤﻞ ﮔﻴﺎه ﻟﻮﺑﻴﺎ ﺳﺒﺰ در ﺗﻨﺶ ﺳﺮﻣﺎ ﻣﻲﺑﺎﺷﺪ.
کلیدواژه ها
 
Title
Investigating the changes of photosynthetic pigment in green bean (Phaseolus vulgaris L.) in response to inoculation of Piriformospora indica and foliar application of paclobutrazol
Authors
Abstract
To study the effect of Piriformospora indica and Paclobutrazol (PBZ) as a plant growth regulator for inducing cold stress tolerance in green beans (Phaseolus vulgaris L.) plant, a greenhouse experiment was performed as a factorial arrangement based completely randomized design with three replications. Treatments consisted of three cold stress levels (control, three and six days cold stress at 5°C), two levels of fungi inoculation (non-inoculated and inoculated with P. indica) and three levels of PBZ (0, 40 and 80 ppm). Eight weeks after planting, green bean plants exposur to cold stress and some morphological and physiological growth characteristics were measured. The results showed that the six days of cold stress particularly reduced aboveground dry weight, the amount of chlorophyll a, b and carotenoids (respectively about 19, 18 and 8%). Generally, in all cold stress levels, the inoculation of P. indica increased the plant dry weight that the largest increase was in six days of cold stress 65%. Spraying of PBZ, particularly 40 ppm, enhanced chlorophyll a and carotenoid as the rate of 5 and 9, respectively. While, PBZ application on inoculated plants significantly reduced dry weight of green beans that the concentration of 40 ppm PBZ reduced to about 17 percent. The results of this study confirmed the positive role of the P. indica and PBZ on growth and improving plant resistance to cold stress in green beans.
Keywords
Chlorophyll, green beans, paclobutrazol, Piriformospora indica