انتقال ژن دفنسین به گیاه کلزا جهت ایجاد مقاومت علیه پاتوژن های قارچی

Pathogens are some of the limiting factor that affects canola in different planting stage and fungal pathogens are the most destructive of them. Application of genetic engineering methods along with biological treatments can be a proper supersede for chemical control which unfortunately is currently the most common method to fight against most of the plant pathogeneses. Among the appropriate useful genes for transmission, are encoding genes of defensins. Defensins are antimicrobial peptides which eliminate the aggressive cells by destroying their membranes.In this study, the cDNA of defensin (RS-AMP1) with radish origin (Raphanus sativus) was cloned in pBI121 expression vector under CaMV35s promoter and then it was approved through implementing PCR and digestion. In order to transfer the cloned gene Agrobacterium tumefaciens of LBA4404 strain and cotyledon explants were used. For recognition of transformed plants obtained, we employed PCR using specific primers.