ارزیابی حضور ژنهای مقاومت در تعدادی از ارقام و لاینهای امیدبخش گندم اقلیم سرد با استفاده از نشانگرهای مولکولی
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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ داﻧﺸﮕﺎه آزاد اﺳﻼﻣﻲ، واﺣﺪ اﺳﻼﻣﺸﻬﺮ،ﮔﺮوه اﺻﻼح ﻧﺒﺎﺗﺎت، اﺳﻼﻣﺸﻬﺮ، اﻳﺮان
2ﻋﻀﻮ ﻫﻴﺌﺖ ﻋﻠﻤﻲ ﻣﺆﺳﺴﻪ ﺗﺤﻘﻴﻘﺎت اﺻﻼح و ﺗﻬﻴﻪ ﻧﻬﺎل و ﺑﺬر، ﻛﺮج، ﺑﻠﻮار ﺷﻬﻴﺪ ﻓﻬﻤﻴﺪه.
3ﻛﺎرﺷﻨﺎس ارﺷﺪ، ﻣﺆﺳﺴﻪ ﺗﺤﻘﻴﻘﺎت اﺻﻼح و ﺗﻬﻴﻪ ﻧﻬﺎل و ﺑﺬر ﻛﺮج، ﺑﻠﻮار ﺷﻬﻴﺪ ﻓﻬﻤﻴﺪه.
چکیده
اﻳﻦ ﺗﺤﻘﻴﻖ ﺑﻪ ﻣﻨﻈﻮر ارزﻳﺎﺑﻲ ﻣﻘﺎوﻣﺖ ﺗﻌﺪادی از ارﻗﺎم و ﻻﻳﻦﻫﺎی اﻣﻴﺪﺑﺨﺶ ﮔﻨﺪم اﻗﻠﻴﻢ ﺳﺮد ﻧﺴﺒﺖ ﺑﻪ زﻧﮓﻫﺎی زرد، ﺳﻴﺎه و ﻗﻬﻮه ای از ﻧﻈﺮ وﺟﻮد ﻳﺎ ﻋﺪم وﺟﻮد ژنﻫﺎی ﻣﻘﺎوﻣﺖ ﺑﺎ اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮﻫﺎی ﻣﻮﻟﻜﻮﻟﻲ اﻧﺠﺎم ﮔﺮدﻳﺪ. در ﻣﺠﻤﻮع 30 رﻗﻢ و ﻻﻳﻦ اﻣﻴﺪ ﺑﺨﺶ اﻗﻠﻴﻢ ﺳﺮد ﻛﺸﻮر از ﻧﻈﺮ ﺣﻀﻮر و ﻳﺎ ﻋﺪم ﺣﻀﻮر ژنﻫﺎی ﻣﻘﺎوﻣﺖ Lr67 ،Lr46-Yr29 ،Lr34 ﺑﺎ اﺳﺘﻔﺎده از 9 ﻧﺸﺎﻧﮕﺮ ﻣﻮﻟﻜﻮﻟﻲ ﭘﻴﻮﺳﺘﻪ ﺑﺎ اﻳﻦ ژنﻫﺎ ﻣﻮرد ﺑﺮرﺳﻲ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻣﻄﺎﻟﻌﻪ ارﻗﺎم و ﻻﻳﻦﻫﺎی ﻣﻮرد ﺑﺮرﺳﻲ ﺑﺎ اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮﻫﺎی ﻣﻮﻟﻜﻮﻟﻲ Sr26#43 ،BE500703 ،CFD71 و Lr25F19 ﻧﺸﺎن داد ﻛﻪ اﻳﻦ ارﻗﺎم و ﻻﻳﻦﻫﺎ ﻓﺎﻗﺪ ژنﻫﺎی ﻣﻘﺎوﻣﺖ ﻣﻮرد ﺑﺮرﺳﻲ ﺑﻮدﻧﺪ. ﻧﺘﺎﻳﺞ ﻛﻠﻲ اﻳﻦ ﺗﺤﻘﻴﻖ ﻧﺸﺎن داد ﻛﻪ اﻛﺜﺮ ارﻗﺎم ﮔﻨﺪم اﻗﻠﻴﻢ ﺳﺮد از ﻧﻈﺮ ژنﻫﺎی ﻣﻮرد ﺑﺮرﺳﻲ ﻓﺎﻗﺪ ﻣﻘﺎوﻣﺖ ﺑﻮده و ﺣﻀﻮر اﻳﻦ ژنﻫﺎی ﻣﻮﺛﺮ ﻓﻘﻂ در ﺗﻌﺪاد ﻣﻌﺪودی از اﻳﻦ ارﻗﺎم و ﻻﻳﻦﻫﺎ ﺗﻌﻴﻴﻦ ﺷﺪ.
کلیدواژه ها
Title
Evaluation of presence resistance genes to trip, leaf and slow rusts in wheat elite cultivars and lines in cold areas using by molecular markers
Authors
Abstract
Abstract
This study was carried out to evaluate adult plant resistance genes to leaf, slow and yellow rusts in some of Iranian wheat cultivars/elite lines in cold areas using linked molecular markers. In total, 30 Iranian wheat cultivars/elite lines in
cold areas were assess for presence or absence of rust resistance genes Lr34 ،Lr46-Yr29 ،Lr67 using 9 linked molecular
markers. Results should that by using molecular markers CFD71،BE500703 ،Sr26#43 و Lr25F19 the majority of genotype lacked these resistance genes and the presence of these genes was determined only in few genotypes.
This study was carried out to evaluate adult plant resistance genes to leaf, slow and yellow rusts in some of Iranian wheat cultivars/elite lines in cold areas using linked molecular markers. In total, 30 Iranian wheat cultivars/elite lines in
cold areas were assess for presence or absence of rust resistance genes Lr34 ،Lr46-Yr29 ،Lr67 using 9 linked molecular
markers. Results should that by using molecular markers CFD71،BE500703 ،Sr26#43 و Lr25F19 the majority of genotype lacked these resistance genes and the presence of these genes was determined only in few genotypes.
Keywords
wheat, wheat rusts, molecular markers, adult plant resistance