تکنولوژی کپسوله کردن جهت نگهداری کوتاه مدت و حفاظت ژرمپلاسم آفتابگردان

somatic embryos derived from the culture of sunflowerʼs immature zygotic embryos on the embryogenesis medium to evaluate their response to different storage periods encapsulated using %3 sodium alginate and 100 mM CaCl2.2H2O supplement with three different matrixes (include distilled water, liquid MS medium and plant growth regulators) and then stored for several period(15, 30, 45 and 60 days) at 4°C. After each storage period, encapsulated and non-encapsulated somatic embryos were cultured on hormone-free MS medium for regeneration and regrowh evaluation. The regrowth ability of encapsulated somatic embryos was affected by the storage duration and the presence or absence of MS nutrients in calcium alginate beads. Percentage response for the conversion of encapsulated and non-encapsulated shoot tips decreased gradually after storage at 4˚C for increasing storage durations that encapsulated vegetative propagules showed a higher resistance to storage at 4˚C than non-encapsulated. Addition of MS nutrients in calcium alginate beads significantly improved encapsulated explants regrowth after storage periods.