ﺑﺮرﺳﻲ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ ﺑﺎﻛﺘﺮیﻫﺎیPGPRﺗﻮﻟﻴﺪ ﻛﻨﻨﺪه اﻛﺴﻴﻦ ﺑﺎ اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮ RAPD
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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ اﺻﻼح ﻧﺒﺎﺗﺎت، داﻧﺸﮕﺎه ﺗﺤﺼﻴﻼت ﺗﻜﻤﻴﻠﻲ ﺻﻨﻌﺘﻲ و ﻓﻨﺎوری ﭘﻴﺸﺮﻓﺘﻪ ﻣﺎﻫﺎن-ﻛﺮﻣﺎن
2ﮔﺮوه ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی، ﭘﮋوﻫﺸﻜﺪه ﻋﻠﻮم ﻣﺤﻴﻄﻲ، ﭘﮋوﻫﺸﮕﺎه ﻋﻠﻮم و ﺗﻜﻨﻮﻟﻮژی ﭘﻴﺸﺮﻓﺘﻪ و ﻋﻠﻮم ﻣﺤﻴﻄﻲ، داﻧﺸﮕﺎه ﺗﺤﺼﻴﻼت ﺗﻜﻤﻴﻠﻲ ﺻﻨﻌﺘﻲ و ﻓﻨﺎوری ﭘﻴﺸﺮﻓﺘﻪ
چکیده
ﻫﻮرﻣﻮن اﻛﺴﻴﻦ ﻳﻜﻲ از ﻣﻬﻤﺘﺮﻳﻦ ﻫﻮرﻣﻮﻧﻬﺎی ﮔﻴﺎﻫﻲ اﺳﺖ ﻛﻪ ﻣﻲﺗﻮاﻧﺪ در رﺷﺪ اﻧﺪاﻣﻬﺎی ﻣﺨﺘﻠﻒ ﮔﻴﺎﻫﻲ ﺑﻮﻳﮋه ﺑﺮای ﺑﻬﺒﻮد ﺳﻴﺴﺘﻢ رﻳﺸﻪای ﮔﻴﺎﻫﺎن زراﻋﻲ ﺗﺤﺖ ﺷﺮاﻳﻂ ﻧﺮﻣﺎل و ﻧﻴﺰ ﺗﻨﺸﻲ ﺑﺴﻴﺎر ﻛﻤﻚ ﻛﻨﻨﺪه ﺑﺎﺷﺪ. ﻣﻄﺎﻟﻌﺎت ﻣﺨﺘﻠﻒ ﻧﺸﺎن دادﻧﺪ ﻛﻪ ﺑﻴﺸﺘﺮ راﻳﺰوﺑﺎﻛﺘﺮﻳﻬﺎ ﺗﻮاﻧﺎﻳﻲ ﺗﻮﻟﻴﺪ ﻫﻮرﻣﻮن اﻛﺴﻴﻦ را دارا ﻫﺴﺘﻨﺪ و اﻳﻦ ﻫﻮرﻣﻮن ﻣﻲﺗﻮاﻧﺪ ﺑﻪ رﺷﺪ ﮔﻴﺎﻫﺎن ﻛﻤﻚ ﻛﻨﺪ. در اﻳﻦ ﻣﻄﺎﻟﻌﻪ 40 راﻳﺰوﺑﺎﻛﺘﺮی ﻣﺨﺘﻠﻒ از راﻳﺰوﺳﻔﺮ ﮔﻴﺎﻫﺎن ﺟﻤﻊآوری ﺷﺪه از ﻣﻨﺎﻃﻖ ﻣﺨﺘﻠﻒ اﻳﺮان از ﻧﻈﺮ ﺗﻮﻟﻴﺪ ﻣﻴﺰان ﻫﻮرﻣﻮن اﻛﺴﻴﻦ ﺑﻪ روش ﺳﺎﻟﻜﻮﻓﺴﻜﻲ ﻣﻮرد آزﻣﻮن ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻧﺘﻴﺠﻪ ﻧﺸﺎن داد ﻛﻪ ﺳﻮﻳﻪﻫﺎی V7 و U4 ﺑﻴﺸﺘﺮﻳﻦ ﻣﻴﺰان ﺗﻮﻟﻴﺪ ﻫﻮرﻣﻮن اﻛﺴﻴﻦ را دارا ﻫﺴﺘﻨﺪ. ﻛﻪ از اﻳﻦ ﺳﻮﻳﻪ ﻣﻲﺗﻮان ﺑﺮای ﻣﻄﺎﻟﻌﺎت ﺑﻌﺪی اﺳﺘﻔﺎده ﻛﺮد. ﻫﻤﭽﻨﻴﻦ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ راﻳﺰوﺑﺎﻛﺘﺮﻳﻬﺎی ﺟﺪاﺳﺎزی ﺷﺪه ﺑﺎ اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮ RAPD ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻗﺮار ﮔﺮﻓﺖ. ﺿﺮﻳﺐ ﺗﺸﺎﺑﻪ ﺑﻴﻦ راﻳﺰوﺑﺎﻛﺘﺮﻳﻬﺎ ﺑﻴﻦ 0 ﺗﺎ 0/55 ﺑﻮد. ﻳﻌﻨﻲ ﺣﺪاﻗﻞ ﺑﺎ اﺳﺘﻔﺎده از ﭘﺮاﻳﻤﺮﻫﺎی ﻣﻮرد اﺳﺘﻔﺎده در اﻳﻦ ﭘﮋوﻫﺶ ﻫﻴﭻ ﺗﺸﺎﺑﻬﻲ ﺑﻴﻦ ﺑﺮﺧﻲ از راﻳﺰوﺑﺎﻛﺘﺮﻳﻬﺎ ﻳﺎﻓﺖ ﻧﺸﺪ ﻛﻪ اﻳﻦ ﻣﻤﻜﻦ اﺳﺖ ﻧﺸﺎﻧﺪﻫﻨﺪه وﺟﻮد ﺟﻨﺴﻬﺎی ﻣﺨﺘﻠﻒ در ﺑﻴﻦ ﺑﺎﻛﺘﺮﻳﻬﺎی ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﺑﺎﺷﺪ. ﻫﻤﭽﻨﻴﻦ ﺗﻤﺎﻣﻲ ﺳﻮﻳﻪ ﻫﺎ در ده ﮔﺮوه ﻣﺨﺘﻠﻒ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. اﻳﻦ ﻧﺘﺎﻳﺞ ﻧﺸﺎن داد ﻛﻪ ﻧﺸﺎﻧﮕﺮ RAPD ﺣﺪاﻗﻞ در اﻳﻦ ﭘﮋوﻫﺶ و ﺑﺮای ﻣﻄﺎﻟﻌﻪ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ ﺑﻴﻦ راﻳﺰوﺑﺎﻛﺘﺮﻳﻬﺎ از ﻛﺎراﻳﻲ ﻧﺴﺒﺘﺎ ﺧﻮﺑﻲ ﺧﻮردار اﺳﺖ.
کلیدواژه ها
Title
Investigation of genetic diversity of Auxin producing Plant Growth Promoting Rhizobacteria (PGPR) by using RAPD markers
Authors
Abstract
Abstract:
Auxin is an important plant hormone that can be useful for root system of crops under normal and stress conditions. Many studies indicated that the most of rhizobacteria are able to produce auxin and this hormone probably can help plant growth. In this study, capability of Auxin production of 40 rhizobacteria isolated from different area of Iran is analyzed by using Salkowski method. The result indicated that two rhizobacteria (V7, U4) can produce more Auxin and can be used in further studies. Genetic diversity between isolated rhizobacteria was studied by using RAPD marker. Similarity coefficient between rhizobacteria was between 0 and 0.55 that means there is no similarity between some rhizobacteria by using this marker and this probably indicated that there are different genus between isolated rhizobacteria. All of rhizobacteria are located in 10 different groups. The result of this study indicated that RAPD marker is useful for studying genetic diversity.
Auxin is an important plant hormone that can be useful for root system of crops under normal and stress conditions. Many studies indicated that the most of rhizobacteria are able to produce auxin and this hormone probably can help plant growth. In this study, capability of Auxin production of 40 rhizobacteria isolated from different area of Iran is analyzed by using Salkowski method. The result indicated that two rhizobacteria (V7, U4) can produce more Auxin and can be used in further studies. Genetic diversity between isolated rhizobacteria was studied by using RAPD marker. Similarity coefficient between rhizobacteria was between 0 and 0.55 that means there is no similarity between some rhizobacteria by using this marker and this probably indicated that there are different genus between isolated rhizobacteria. All of rhizobacteria are located in 10 different groups. The result of this study indicated that RAPD marker is useful for studying genetic diversity.
Keywords
PGPR, Auxin, genetic diversity, RAPD markers