برطرف کردن خفتگی بذر در گیاه همیشک از طریق کشت بافت

Danae racemosa is a beautiful and evergreen shrub of Liliaceae family which has rhizome and cladodes. This plant is native of Iran and is endangered due to its slow-growing in natural environment and abundant harvesting for flower arrangements. It has also medicinal properties such as antioxidant and antithrombic uses in traditional medicine and pharmacy. Because of seed dormancy, seed germination of Danae racemosa, takes about a year, and the emergence of the first and second leaf occurs in the second and third year of seed culture. The purpose of this study is introducing a method for rapid proliferation of Danae racemosa via in vitro seed culture. Experiments were performed on immature and semi-mature seed so that it was studied the effect of different treatments such as cold, dark and gibberellic acid on in vitro seed germination. The results showed that seed treatment with cold stratification(3-4degrees C) for 10-14 days and followed by seed culture in Murashige and skoog medium supplemented with gibberellic acid in dark condition, is suitable for breaking of seed dormancy of Danae racemosa.