تعیین درصد بذربردی ویروس موزایئک معمولی لوبیا و ویروس موزائیک خیار در ارقام لوبیا خشک

Bean common mosaic Potyvirus (BCMV), a member of the viral family Potiviridae, and cucumber mosaic Cucumovirus (CMV), from the family Bromovoridae, are responsible for severe crop loss in varieties of beans. Since viral transmission through the seeds could introduce the primary inoculums in new season, and it is known that planting of infected seeds has important role in distribution and being epidemic, the aim of this study was to assess the level of seed transmissibility of BCMV and CMV in three genotypes of beans (pinto, red and navy). For this, 50 seeds from each of the red, pinto and navy varieties were collected from markets, and were grown in greenhouse. Infectivity to the BCMV and CMV in these seeds was tested by ELISA and RT-PCR in two-week old plants. The percentage of the infected plants was equal to the percentage of infected seeds. The infected plants let to maturation and their seeds were collected and were planted and their infectivity to both viruses was determined as being described. This procedure was done for three generations. The results showed that the sensitivity to and seed transmissibility of BCMV and CMV differed in the three genotypes in which being cowpea the most susceptible to CMV, while the red and pinto varieties were more susceptible to BCMV. The levels of seed transmissible of CMV and BCMV in these varieties were the same as those of susceptibility to the viruses.