بررسی تنوع ژنتیکی جمعیت های جوی وحشی و زراعی براساس چند شکلی پروتئین های ذخیره ای

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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی دﻛﺘﺮی داﻧﺸﮕﺎه آزاد اﺳﻼﻣﻲ واﺣﺪ ﻋﻠﻮم و ﺗﺤﻘﻴﻘﺎت ﺗﻬﺮان
2اﺳﺘﺎد ﭘﮋوﻫﺸﻜﺪه ﻣﺆﺳﺴﻪ ﺗﺤﻘﻴﻘﺎت ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﻛﺸﺎورزی ﻛﺮج
3اﺳﺘﺎد داﻧﺸﮕﺎه ﺗﺒﺮﻳﺰ
4داﻧﺸﻴﺎر داﻧﺸﮕﺎه ﭘﻴﺎم ﻧﻮر ﺗﻬﺮان
چکیده
اﻣﺮوزه ﺑﺼﻮرت ﻋﻤﻮﻣﻲ ﭘﺬﻳﺮﻓﺘﻪ ﺷﺪه ﻛﻪ ﺟﻮی زراﻋﻲ از Hordeum vulgare L. subsp. Spontaneumﻣﻨﺸﺎء ﮔﺮﻓﺘﻪ اﺳﺖ، ﺑﺎ اﻳﻦ وﺟﻮد ﻫﻨﻮز دﻗﻴﻘﺎً ﻣﺸﺨﺺ ﻧﻴﺴﺖ ﻛﻪ ﻓﺮآﻳﻨﺪ اﻫﻠﻲ ﺷﺪن اﻳﻦ ﮔﻴﺎه ﻓﻘﻂ در ﻣﻨﻄﻘﻪ ﻫﻼل ﺣﺎﺻﻠﺨﻴﺰ اﺗﻔﺎق اﻓﺘﺎده و ﻳﺎ اﻳﻦ ﻓﺮآﻳﻨﺪ در ﻧﻘﺎط ﻣﺨﺘﻠﻔﻲ از ﺟﻬﺎن ﺑﻪ ﺻﻮرت ﻫﻤﺰﻣﺎن ﺑﻪ وﻗﻮع ﭘﻴﻮﺳﺘﻪ اﺳﺖ. در ﺗﺤﻘﻴﻖ ﺣﺎﺿﺮ ﺑﻪ ﻣﻨﻈﻮر ﺗﻌﻴﻦ ﻣﺮﻛﺰ ﻳﺎ ﻣﺮاﻛﺰ اﺻﻠﻲ ﺗﻨﻮع و اﻫﻠﻲ ﺷﺪن ﮔﻴﺎه ﺟﻮ، 266 ﺟﻮی وﺣﺸﻲ H. spontaneum از ﻣﻨﺎﻃﻘﻲ واﻗﻊ در ﻫﻼل ﺣﺎﺻﻠﺨﻴﺰ و ﺧﺎرج از آن ﺑﻪ ﻫﻤﺮاه 44 ﻧﻤﻮﻧﻪ ﺟﻮی زراﻋﻲ از ﻧﻘﺎط ﻣﺨﺘﻠﻒ دﻧﻴﺎ از ﻃﺮﻳﻖ ﺗﺠﺰﻳﻪ ﭘﺮوﺗﺌﻴﻦ ﻫﺎی ذﺧﻴﺮه ای ﻣﻮرد ﺑﺮرﺳﻲ ﻗﺮار ﮔﺮﻓﺖ. ﺑﺮای ﻫﻮردﺋﻴﻦ ﻫﺎی C ،D و B ﺑﻪ ﺗﺮﺗﻴﺐ 12 ، 21 و 20 اﻟﮕﻮی ﻣﺘﻔﺎوت ﻣﺸﺎﻫﺪه ﺷﺪ. ﭘﺎراﻣﺘﺮﻫﺎی ژﻧﺘﻴﻜﻲ، ﻧﻈﻴﺮ ﺗﻌﺪاد اﻟﮕﻮ و ﺷﺎﺧﺺ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ ﻧﺸﺎن داد اﺣﺘﻤﺎﻻً ﻣﻨﻄﻘﻪ ﻫﻼل ﺣﺎﺻﻠﺨﻴﺰ و ﺧﺼﻮﺻﺎً ﻧﻮاﺣﻲ ﺟﻨﻮب ﻏﺮب اﻳﺮان، ﺷﻤﺎل ﻋﺮاق، ﺟﻨﻮب ﺷﺮق ﺗﺮﻛﻴﻪ و اردن ﻣﺮاﻛﺰ اﺻﻠﻲ ﺗﻨﻮع ﮔﻴﺎه ﺟﻮی وﺣﺸﻲ H. spontaneum ﻣﻲ ﺑﺎﺷﻨﺪ. ﺑﺎ اﻳﻦ ﺣﺎل ﻧﻮاﺣﻲ ﺷﻤﺎل اﻓﺮﻳﻘﺎ و آﺳﻴﺎی ﺷﺮﻗﻲ ﻣﻲ ﺗﻮاﻧﻨﺪ ﺑﻪ ﻋﻨﻮان ﻣﺮاﻛﺰ ﺛﺎﻧﻮﻳﻪ ﺗﻨﻮع اﻳﻦ ﮔﻴﺎه ﻣﻄﺮح ﺑﺎﺷﻨﺪ.از ﺳﻮی دﻳﮕﺮ ﺑﺮرﺳﻲ ﻓﻮاﺻﻞ ژﻧﺘﻴﻜﻲ ﺟﻤﻌﻴﺖ ﻫﺎ ﻧﺸﺎن داد ﻛﻪ ژﻧﻮﺗﻴﭗ ﻫﺎی ﺟﻮی وﺣﺸﻲ ﺷﻤﺎل ﻋﺮاق و ﺟﻨﻮب ﺷﺮق ﺗﺮﻛﻴﻪ ﻧﺰدﻳﻜﺘﺮﻳﻦ ﺟﻤﻌﻴﺖ ﻫﺎ ﺑﻪ ﻧﻤﻮﻧﻪ ﻫﺎی زراﻋﻲ اﻣﺮوزی ﺑﻮدﻧﺪ. اﻳﻦ ﻧﺘﻴﺠﻪ ﻧﺸﺎﻧﮕﺮ آﻧﺴﺖ ﻛﻪ اﻳﻦ ﻧﻮاﺣﻲ ﻣﺤﺘﻤﻞ ﺗﺮﻳﻦ ﻣﻨﺎﻃﻖ ﺑﺮای اﻫﻠﻲ ﺷﺪن ﮔﻴﺎه ﺟﻮ ﺑﺤﺴﺎب ﻣﻲ آﻳﻨﺪ.
کلیدواژه ها
 
Title
Assessment of genetic diversity of wild and cultivated barley populations based on storage proteins polymorphis
Authors
Abstract
Although, It is now generally accepted that domestication of barley is assumed to have taken place from Hordeum vulgare L. subsp. Spontaneum, however, it is not clear whether cultivated barley is of monophyletic origin or the domestication events happened in other parts of the world besides the Fertile Crescent. In this study in order to determine the center or centers of diversity and domestication of barley, 266 wild barley H. spontaneum in the Fertile Crescent region and beyond, with 44 samples of barley crops around the world were examined by Storage proteins . For Hordeins of D, C and B, were observed 12 , 21 and 20 different patterns respectively. Genetic parameters , such as number patterns and genetic diversity index showed Fertile Crescent region especially southern West of Iran, north of Iraq , South East of Turkey and Jordan were the major centers of plant diversity wild barley H. spontaneum. However, parts of North Africa and East Asia could be used as a secondary center of diversity of this plant are discussed. The study of genetic distances showed that wild barley genotypes of north of Iraq and South East of Turkey, were the nearest populations to the modern cultivated samples. This result indicates that this areas are considered the most likely for barley domestication.
Keywords
domestication, genetic diversity, wild barley H.Spontaneum, Hordeins Polymorphism, Fertile Crescent