بررسی امکان بهبود کالوس دهی و باززایی گیاه نیشکر در شرایط درونشیشهای

Abstract
Due to its high genetic diversity, sugarcane (Saccharum officinalis L.) is grown and propagated in a vegetative manner. In this respect, employing tissue culture techniques could be a very efficient way to produce and preserve, such a genetic diversity in sugarcane. In this regard, in order to callus induction and plant regeneration of two commercial cultivars, CP69-1062 (A) and CP48-103 (B), specific small tissues were prepared by cutting down the base of the plantlet’s stem and then were cultured on MS medium containing with 5 different concentration of 2,4-D with a spectrum of 0, 0.5, 1, 1.5, and 2 mg/L. The growing tissues have been evaluated following data analysis after 4 weeks,. The obtained data on cultivar A showed that 2,4-D in concentration of 1.5 mg/L could induce the highest amount of callus induction and planlet regeneration. Moreover, In cultivar B, this phytohormone could induce the highest amount of callus induction in 1.5 mg/L, and highest amount of regeneration in concentration of 0.5 mg/L. The obtained results emphasized that this approach could decrease the needed time for callus induction and plantlet regeneration in sugarcane. The results were utilized for making manipulated communities and selecting the proper plants with a high tolerance to environmental stresses.