اﺛﺮ ﻣﺘﻴﻞ ﺟﺎﺳﻤﻮﻧﺎت و ﺳﺎﻟﻴﺴﻴﻠﻴﻚ اﺳﻴﺪ ﺑﺮ ﻣﺤﺘﻮای اﺳﺘﻮﻳﻮزاﻳﺪ اﺳﺘﻮﻳﺎ ( Stevia rebaudiana Bert ) در ﺷﺮاﻳﻂ درون ﺷﻴﺸﻪای

Abstract
Stevioside, an ent-kaurene type of diterpenoid glycoside, is a natural sweetener extracted from leaves of stevia rebaudiana Bertoni a perennial herb of the asteraceae family that can be used as substitute of artificial sweeteners for diabetic patients. Elicitors could be used as enhancers of plant secondary metabolites synthesis and could play an important role in biosynthetic pathways to production of commercially important compounds. This investigation aimed to study the improving effect of methyl jasmonate and salicylic acid foliar spray application (0, 0.5 and 1 mM) on stevioside content of stevia in in-vitro conditions. Shoot tip and nodal segment were used as explants and then were cultured on Murashige and Skoog medium. After 30 days, both of the elicitor’s effects on the production of stevioside were studied. Plantlets were harvested after 14 days and high-performance liquid chromatography was used to assess and quantification of stevioside content. Experiment was carried out as factorial based on completely randomized design (CRD) with two replications. The results showed that plantlets of stevia treated with methyl jasmonate and salicylic acid increased the content of stevioside compared to non-treated plantlets. It was also noticed that foliar spray with methyl jasmonate had higher effect on stevioside content than salicylic acid.