اﻟﻘﺎی رﻳﺸﻪ ﻣﻮﻳﻴﻦ در ﮔﻴﺎه داروﻳﻲ ﺗﺸﻨﻪداری ( Scrophularia striata Boiss. )
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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﻛﺸﺎورزی،ﮔﺮوه اﺻﻼح و ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﮔﻴﺎﻫﻲ، داﻧﺸﻜﺪه ﻛﺸﺎورزی،داﻧﺸﮕﺎه ارومیه
2اﺳﺘﺎدﻳﺎر، ﮔﺮوه اﺻﻼح و ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﮔﻴﺎﻫﻲ،داﻧﺸﻜﺪه ﻛﺸﺎورزی، داﻧﺸﮕﺎه اروﻣﻴﻪ
3اﺳﺘﺎدﻳﺎر،ﮔﺮوه ﺗﺤﻘﻴﻘﺎت ﻛﺮوﻣﺎﺗﻮﮔﺮاﻓﻲ،ﺟﻬﺎد داﻧﺸﮕﺎﻫﻲ آذرﺑﺎﻳﺠﺎن ﻏﺮﺑﻲ، ارومیه
چکیده
ﮔﻴﺎه ﺗﺸﻨﻪداری (Scrophularia striata Boiss) ﻣﺘﻌﻠﻖ ﺑﻪ ﺧﺎﻧﻮاده ﮔﻞ ﻣﻴﻤﻮﻧﻲ اﺳﺖ ﻛﻪ ﺑﻪﻃﻮر ﮔﺴﺘﺮدهای در ﻫﻤﻪ ﻧﻘﺎط ﺑﻪ ﺧﺼﻮص در ﺑﺨﺶ ﺷﻤﺎل ﺷﺮﻗﻲ اﻳﺮان ﭘﺮاﻛﻨﺪه ﺷﺪه اﺳﺖ. اﻳﻦ ﮔﻴﺎه داروﻳﻲ دارای ﻣﺘﺎﺑﻮﻟﻴﺖﻫﺎی ﺛﺎﻧﻮﻳﻪ ﻣﻬﻤﻲ ﻣﺎﻧﻨﺪ ﻟﻴﻨﺎﻟﻮل، ﺟﺮﻣﺎﻛﺮﻳﻦ دی، ﺑﺘﺎ داﻣﺎﺳﻜﻮن اﺳﺖ. ﻟﻴﻨﺎﻟﻮل ﻋﻼوه ﺑﺮ اﻳﻦﻛﻪ اﺛﺮات ﺿﺪﻣﻴﻜﺮوﺑﻲ و ﺿﺪوﻳﺮوﺳﻲ دارد، ﺑﺎزدارﻧﺪه رﺷﺪ ﺗﻮﻣﻮرﻫﺎی ﺳﺮﻃﺎﻧﻲ اﺳﺖ. ﻣﺘﺎﺑﻮﻟﻴﺖ ﻫﺎی ﺛﺎﻧﻮﻳﻪ در ﻣﻘﺎدﻳﺮ ﻛﻢ و در ﻣﺮﺣﻠﻪ ﺧﺎﺻﻲ از زﻧﺪﮔﻲ ﮔﻴﺎه ﺗﻮﻟﻴﺪ ﻣﻲﺷﻮﻧﺪ. ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ارزش داروﻳﻲ آﻧﻬﺎ، ﻣﻲﺗﻮان ﻣﻴﺰان ﺗﻮﻟﻴﺪ اﻳﻦ ﺗﺮﻛﻴﺒﺎت را ﺑﺎ اﺳﺘﻔﺎده از روشﻫﺎی زﻳﺴﺖ ﻓﻨﺎوری اﻓﺰاﻳﺶ داد. ااﻟﻘﺎی رﻳﺸﻪ ﻣﻮﻳﻴﻦ از ﺳﻴﺴﺘﻢﻫﺎی ﻣﻨﺎﺳﺐ ﺑﺮای ﺗﻮﻟﻴﺪ ﻣﺘﺎﺑﻮﻟﻴﺖﻫﺎی ﺛﺎﻧﻮﻳﻪ اﺳﺖ. در اﻳﻦ ﺗﺤﻘﻴﻖ از ﺑﺎﻛﺘﺮی Agrobacterium rhizogenes ﺳﻮﻳﻪ A13ﺑﻪ ﻣﻨﻈﻮر اﻟﻘﺎی رﻳﺸﻪ ﻣﻮﻳﻴﻦ در ﮔﻴﺎه ﺗﺸﻨﻪداری اﺳﺘﻔﺎده ﺷﺪ. ﻫﻤﭽﻨﻴﻦ اﺛﺮ اﺳﻴﺪ ﺳﻴﻨﺎﻣﻴﻚ (CA)ﺑﻪ ﻋﻨﻮان ﻋﺎﻣﻞ اﻟﻘﺎ ﻛﻨﻨﺪه ﺑﻴﺎن ژنﻫﺎی vir ﺑﺎﻛﺘﺮی ﺑﺎ دو ﻏﻠﻈﺖ )150µMو 100 ﺑﺮای اﻓﺰاﻳﺶ ﻛﺎراﻳﻲ اﻟﻘﺎی رﻳﺸﻪﻫﺎی ﻣﻮﻳﻴﻦ ﻣﻄﺎﻟﻌﻪ ﺷﺪ. ﺗﺎﻳﻴﺪ ﻣﻮﻟﻜﻮﻟﻲ رﻳﺸﻪ ﻫﺎی ﻣﻮﻳﻴﻦ ﺑﺎ واﻛﻨﺶ زﻧﺠﻴﺮهای ﭘﻠﻲ ﻣﺮاز ﺑﺮای ﺗﻜﺜﻴﺮ ﺑﺨﺸﻲ از ژنﻫﺎی rolAو rolB اﻧﺠﺎم ﮔﺮدﻳﺪ.ﺑﺎ اﺳﺘﻔﺎده از CA ﺑﺎ ﻏﻠﻈﺖ 100µMﺑﻴﺸﺘﺮﻳﻦ ﺗﻌﺪاد رﻳﺸﻪ اﻟﻘﺎﻳﻲ( 25 رﻳﺸﻪ) ﺑﺪﺳﺖ آﻣﺪ. رﻳﺰﻧﻤﻮﻧﻪ ﻫﺎی ﻣﺨﺘﻠﻒ ﻫﻴﭙﻮﻛﻮﺗﻴﻞ، ﻛﻮﺗﻴﻠﺪون، ﺑﺮگ، دﻣﺒﺮگ( ﺑﻪ ﻣﻨﻈﻮر ارزﻳﺎﺑﻲ ﭘﺘﺎﻧﺴﻴﻞ آﻧﻬﺎ در اﻟﻘﺎی رﻳﺸﻪ ﻣﻮﻳﻴﻦ ﻣﻮرد ﺑﺮرﺳﻲ ﻗﺮار ﮔﺮﻓﺖ. رﻳﺰﻧﻤﻮﻧﻪ ﻫﻴﭙﻮﻛﻮﺗﻴﻞ ﺑﺎ ﻣﻴﺎﻧﮕﻴﻦ 4,5 رﻳﺸﻪ در ﻫﺮ رﺑﺰﻧﻤﻮﻧﻪ ﺑﻴﺸﺘﺮﻳﻦ ﺗﻌﺪاد رﻳﺸﻪ اﻟﻘﺎﻳﻲ را داﺷﺖ.اﻳﻦ ﺗﺤﻘﻴﻖ اوﻟﻴﻦ ﮔﺰارش درﺑﺎره اﻟﻘﺎی رﻳﺸﻪ ﻣﻮﻳﻴﻦ در ﮔﻴﺎه ﺗﺸﻨﻪداری اﺳﺖ.
کلیدواژه ها
Title
Hairy root induction in the medicinal plant,ScrophulariastriataBoiss.
Authors
Abstract
Scrophularia striata Boiss. belongs to the Scrophulariaceae family is a small, many branched perennial herb which is widely distributed in all parts, especially in the northeastern part of Iran.This medicinal plants contains the important secondary metabolites such as linalool, βdamasconeo and germacrene D. In addition to antimicrobial and antiviral activities of S.striata extract, itsanti-cancer activity was also reported.Secondary metabolites are produced in small amounts at a specific stage of life cycle of plants.The production of secondary metabolites can be improvedvia the biotechnological methods such as hairy root system. In this study, Agrobacterium rhizogenesstrain A13 was used to induce hairy roots.Different explants (leaf, hypocotyl,cotyledon, and seedling) were used to assess their potential in hairy root induction.The effect of Cinnamicacid (CA) with two different concentrations (100 and 150 µM) on efficiency of hairy root induction was also evaluated.Transgenic status of hairy root lines was confirmedby PCR analysisusing rolA-B genes-specific primers.Higher rate of hairy root intion was optained with 100 µMCA.The highest number of hairy roots (4.5 roots per explant) was observed with hypocotyl explants.This study is the first report on hairy root induction in S.striata.
Keywords
Agrobactriumrhizogenes, cinnamic acid, explant, hairy root induction, Scrophulariastriata