ژن ﻓﺴﻔﻮﻣﺎﻧﻮز اﻳﺰوﻣﺮاز ( pmi )ﺑﻪ ﻋﻨﻮان ﻳﻚ ﻧﺸﺎﻧﮕﺮ اﻧﺘﺨﺎﺑﻲ ﺑﺮای ﺗﺮارﻳﺨﺘﻲ ﺗﻮﺗﻮن ﺑﻮاﺳﻄﻪ آﮔﺮوﺑﺎﻛﺘﺮﻳﻮم

A positive selection system using the phosphomannose isomerase (pmi) gene was employed for Agrobacterium tumefaciens mediated transformation of Nicotiana tabacum. In the present study, various concentrations of mannose (0, 10, 20, 30 g/l) in combination with sucrose (0, 5 g/l) were test to determine the efficiency of mannose based selection system for the screen of putative transformants of tobacco plant. Results showed that the optimal concentration of mannose for the selecting of the transformed cells were 20 g/l without sucrose, because at this concentration the least plant growth was observed for the non-transformed cells in compared with cells transformed with the pmi gene. Transgenic status of selected plantlets was confirmed using PCR. Our results indicated that mannose based selection in tobacco was more efficient, as a selection efficiency of 94.56% and rate of transformation of 21.75% were achieved.