ارزﻳﺎﺑﻲ ژﻧﺘﻴﻜﻲ ﭼﻨﺪﻳﻦ ﻻﻳﻦ Ricinus Communis L ﺑﺎ اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮ ﻣﻮﻟﻜﻮﻟﻲ
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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ ﺑﻴﻮﺗﻜﻨﻮﻟﻮزی ﻛﺸﺎورزی ،ﮔﺮوه ﻛﺸﺎورزی ، داﻧﺸﮕﺎه آزاد اﺳﻼﻣﻲ واﺣﺪ داﻣﻐﺎن ، داﻣﻐﺎن ، اﻳﺮان.
2ﻋﻀﻮ ﻫﻴﺌﺖ ﻋﻠﻤﻲ ﺳﺎزﻣﺎن ﭘﮋوﻫﺶ ﻫﺎی ﻋﻠﻤﻲ ﺻﻨﻌﺘﻲ اﻳﺮان ، ﮔﺮوه ﺗﺤﻘﻴﻘﺎت ﮔﻴﺎﻫﺎن داروﻳﻲ ، ﺗﻬﺮان ، اﻳﺮان
3ﮔﺮوه ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﻛﺸﺎورزی ، داﻧﺸﻜﺪه ﻛﺸﺎورزی ، داﻧﺸﮕﺎه آزاد اﺳﻼﻣﻲ واﺣﺪ داﻣﻐﺎن ، داﻣﻐﺎن ، اﻳﺮان
چکیده
ﻛﺮﭼﻚ ﺑﺎ ﻧﺎم ﻋﻠﻤﻲ Ricinus Communis L. از ﺟﻤﻠﻪ ﮔﻴﺎﻫﺎن داروﻳﻲ ﻣﻬﻢ ﻣﻲ ﺑﺎﺷﺪ.در اﻳﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﻲ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ ﮔﻴﺎه ﻛﺮﭼﻚ از ﻧﺸﺎﻧﮕﺮ ﻣﻮﻟﻜﻮﻟﻲ RAPD اﺳﺘﻔﺎده ﺷﺪه اﺳﺖ. اﺳﺘﺨﺮاج DNA از ارﻗﺎم ﻛﺮﭼﻚ ﺑﻪ روش Delaporta اﻧﺠﺎم ﺷﺪه و ﺑﺎ 5 ﻋﺪد آﻏﺎزﮔﺮ ﺗﺼﺎدﻓﻲ ﺗﻜﺜﻴﺮ ژﻧﻮم ﻧﻤﻮﻧﻪﻫﺎ اﻧﺠﺎم ﺷﺪ. ﺑﺎ اﺳﺘﻔﺎده از ﺿﺮﻳﺐ ﺗﺸﺎﺑﻪ ﺟﺎﻛﺎرد و ﻧﻲ ﻣﺎﺗﺮﻳﺲ ﺗﺸﺎﺑﻪ ﺗﺸﻜﻴﻞ ﺷﺪ. دﻧﺪروﮔﺮام ﺑﺮاﺳﺎس روش UPGM ارﻗﺎم را در 5 ﮔﺮوه ﻣﺘﻤﺎﻳﺰ ﻗﺮار داد. ﺑﻴﺸﺘﺮﻳﻦ ﺗﺸﺎﺑﻪ ﺑﻴﻦ ارﻗﺎم 7 و 9 ﻣﺸﺎﻫﺪه ﺷﺪ ﻛﻪ ﻣﻌﺎدل 0/576 ﻣﻲﺑﺎﺷﺪو ﻛﻤﺘﺮﻳﻦ ﻣﻴﺰان ﺗﺸﺎﺑﻪ ﺑﻴﻦ ارﻗﺎم 8 و 5 و ﻧﻴﺰ ارﻗﺎم 5 و 6 دارا ﻫﺴﺘﻨﺪ ﻛﻪ ﺑﺮاﺑﺮﺑﺎ 0/139 ﻣﻲ ﺑﺎﺷﺪ. ﻧﺘﺎﻳﺞ ﺑﺪﺳﺖ آﻣﺪه ﻧﺸﺎن ﻣﻲدﻫﺪ ﺑﻴﺸﺘﺮﻳﻦ ﺗﻌﺪاد ﺑﺎﻧﺪ ﻣﺮﺑﻮط ﺑﻪ آﻏﺎزﮔﺮ Oligo20 )45 ﺑﺎﻧﺪ و ﻛﻤﺘﺮﻳﻦ ﺗﻌﺪاد ﺑﺎﻧﺪ ﺑﻪ دﺳﺖ آﻣﺪه ﻣﺮﺑﻮط ﺑﻪ آﻏﺎزﮔﺮ Oligo7)17 ﺑﺎﻧﺪ اﺳﺖ.
کلیدواژه ها
Title
Key words : castor bean , genetic diversity , molecular marker , RAPD
Authors
Abstract
Ricinus Communis L is on of the important plants . In this study to avow genetic diversity in castor oil plant have been used RAPD marker PCR-based. DNA extracted from castor varieties of Ricinus Communis L. performed by delaporta method with 5 random primers.
. Dendrogram distinguished castor varieties in 5 groups on the basis of UPGMI method. The greatest similarity between Figures 7 and 9, which is equivalent to 576 / 0 of . , And the lowest similarity Figures 8 and 5 and 5 and 6 , which is equivalent to 139 / 0. Results were shown maximum band was for primer oligo20 (45 band) and minimum band was for primer oligo7 (17 band ).
. Dendrogram distinguished castor varieties in 5 groups on the basis of UPGMI method. The greatest similarity between Figures 7 and 9, which is equivalent to 576 / 0 of . , And the lowest similarity Figures 8 and 5 and 5 and 6 , which is equivalent to 139 / 0. Results were shown maximum band was for primer oligo20 (45 band) and minimum band was for primer oligo7 (17 band ).