ﺗﺄﺛﻴﺮ ﻏﻠﻈﺖﻫﺎی ﻣﺨﺘﻠﻒ ﻧﻴﻜﻞ ﺑﺮ وﻳﮋﮔﻲﻫﺎی ﺑﻴﻮﺷﻴﻤﻴﺎﻳﻲ ﮔﻴﺎﻫﭽﻪ ﻫﺎی ذرت ( .Zea mays L )
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اصل مقاله (143.95 K)
عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1دانشجوی دکتری زراعت، دانشگاه گیلان
2دانش آموخته کارشناسی ارشد زراعت، دانشگاه گیلان
3استادیار گروه زراعت و اصلاح نباتات، دانشگاه گیلان
چکیده
ﺑﺮای ﺑﺮرﺳﻲ ﺗﺄﺛﻴﺮ ﻏﻠﻈﺖﻫﺎی ﻣﺨﺘﻠﻒ ﻧﻴﻜﻞ ﺑﺮ وﻳﮋﮔﻲﻫﺎی ﺑﻴﻮﺷﻴﻤﻴﺎﻳﻲ ﮔﻴﺎهﭼﻪﻫﺎی ذرت ).Zea mays L(، آزﻣﺎﻳﺸﻲ ﺑﻪ ﺻﻮرت ﻃﺮح ﻛﺎﻣﻼً ﺗﺼﺎدﻓﻲ ﺑﺎ ﺳﻪ ﺗﻜﺮار در داﻧﺸﻜﺪه ﻋﻠﻮم ﻛﺸﺎورزی داﻧﺸﮕﺎه ﮔﻴﻼن اﻧﺠﺎم ﺷﺪ. ﺑﺬرﻫﺎی رﻗﻢ ﺳﻴﻨﮕﻞ ﻛﺮاس 704 در ﮔﻠﺪانﻫﺎﻳﻲ ﺣﺎوی ﻣﺨﻠﻮﻃﻲ از ﻣﺎﺳﻪ و ﭘﺮﻟﻴﺖ ﻛﺎﺷﺘﻪ و ﺳﭙﺲ ﺗﺎ رﺳﻴﺪن ﺑﻪ ﻣﺮﺣﻠﻪ ﭼﻬﺎر ﺑﺮﮔﻲ ﺑﺎ اﺳﺘﻔﺎده از ﻣﺤﻠﻮل ﻫﻮﮔﻠﻨﺪ ﺗﻐﺬﻳﻪ ﺷﺪﻧﺪ. ﭘﺲ از آن، ﺗﻴﻤﺎرﻫﺎی ﻧﻴﻜﻞ ﺻﻔﺮ، 50 و 100 ﻣﻴﻜﺮوﻣﻮﻻر( از ﻣﻨﺒﻊ ﺳﻮﻟﻔﺎت ﻧﻴﻜﻞ ﺑﺎ ﺷﺶ ﻣﻮﻟﻜﻮل آب )NiSO4.6H2O( ﺑﻪ ﻣﺪت 10 روز ﺑﺮ روی ﮔﻠﺪانﻫﺎ اﻋﻤﺎل ﺷﺪﻧﺪ. دو ﻧﻤﻮﻧﻪ ﻳﻜﻲ ﭼﻬﺎر و دﻳﮕﺮی ﻫﻔﺖ روز ﭘﺲ از ﭘﺎﻳﺎن اﻋﻤﺎل ﺗﻴﻤﺎرﻫﺎ ﺑﺮداﺷﺘﻪ ﺷﺪ و ﻏﻠﻈﺖ ﻣﺎﻟﻮن دی آﻟﺪﺋﻴﺪ و آﻧﺰﻳﻢﻫﺎی آﻧﺘﻲ اﻛﺴﻴﺪاﻧﻲ ﻛﺎﺗﺎﻻز و ﭘﺮاﻛﺴﻴﺪاز آﻧﻬﺎ اﻧﺪازه ﮔﻴﺮی ﺷﺪ. ﻧﺘﺎﻳﺞ ﻧﺸﺎن داد ﻛﻪ ﻣﻘﺪار ﻧﻴﻜﻞ اﺛﺮ ﺑﺴﻴﺎر ﻣﻌﻨﻲ داری ﺑﺮ ﻏﻠﻈﺖ ﻣﺎﻟﻮن دی آﻟﺪﺋﻴﺪ و آﻧﺰﻳﻢﻫﺎی آﻧﺘﻲ اﻛﺴﻴﺪاﻧﻲ ﻛﺎﺗﺎﻻز و ﭘﺮاﻛﺴﻴﺪاز ﻧﻤﻮﻧﻪ ﻫﺎی ﻣﻮرد ﻣﻄﺎﻟﻌﻪ داﺷﺘﻪ اﺳﺖ. ﻣﻘﺎﻳﺴﻪ ﻣﻴﺎﻧﮕﻴﻦﻫﺎ ﻧﺸﺎن داد ﻛﻪ ﻧﻴﻜﻞ ﺑﺮ ﻓﻌﺎﻟﻴﺖ آﻧﺰﻳﻢﻫﺎی آﻧﺘﻲاﻛﺴﻴﺪاﻧﻲ ﻛﺎﺗﺎﻻز و ﭘﺮاﻛﺴﻴﺪاز اﺛﺮ ﻣﻨﻔﻲ داﺷﺘﻪ و ﺳﺒﺐ ﻛﺎﻫﺶ آﻧﻬﺎ ﺷﺪه اﺳﺖ. ﻧﻴﻜﻞ ﺑﺮ ﻣﺤﺘﻮای ﻣﺎﻟﻮن دی آﻟﺪﺋﻴﺪ ﻛﻪ ﻧﺸﺎن دﻫﻨﺪه ﻣﻴﺰان ﭘﺮاﻛﺴﻴﺪاﺳﻴﻮن ﻟﻴﭙﻴﺪی اﺳﺖ، اﺛﺮ ﻣﺜﺒﺖ داﺷﺖ.
کلیدواژه ها
Title
Effect of different concentrations of Ni on biochemistry characteristics of maize
(Zea mays L.) Seedlings
Authors
Abstract
bstract
In order to investigate the effects of different concentrations of Ni on biochemical characteristics of maize (Zea mays L.) seedlings, a CRD based pot experiment with three replications was carried out at Faculty of Agricultural Sciences, University of Guilan. Seeds were sown in pots already filled with a mixture of sand and perlite (1:1) and irrigated by hoagland’s solution until seedlings reached to 4 leaves stages. Three solutions with Ni concentration of 0, 50 and 100 µM were considered as treatments and added to pots at the stage and continued up to 10 days. Nickel sulphate (NiSO4.6H2O) was used to prepare solutions. Two samples were taken in 4th and 7th days after four leaves stage. Results showed that biochemistry characteristics were significant with probability level of 1%. Result of the comparison of the mean showed the negative effect of Ni on antioxidant enzyme activity of catalase and peroxidase and cause of they decreased and increasing the concentration of Malone aldehyde was happened that represents lipid peroxidation.
In order to investigate the effects of different concentrations of Ni on biochemical characteristics of maize (Zea mays L.) seedlings, a CRD based pot experiment with three replications was carried out at Faculty of Agricultural Sciences, University of Guilan. Seeds were sown in pots already filled with a mixture of sand and perlite (1:1) and irrigated by hoagland’s solution until seedlings reached to 4 leaves stages. Three solutions with Ni concentration of 0, 50 and 100 µM were considered as treatments and added to pots at the stage and continued up to 10 days. Nickel sulphate (NiSO4.6H2O) was used to prepare solutions. Two samples were taken in 4th and 7th days after four leaves stage. Results showed that biochemistry characteristics were significant with probability level of 1%. Result of the comparison of the mean showed the negative effect of Ni on antioxidant enzyme activity of catalase and peroxidase and cause of they decreased and increasing the concentration of Malone aldehyde was happened that represents lipid peroxidation.