اثر ﺳﺎﻟﻴﺴﻴﻠﻴﻚ اﺳﻴﺪ ﺑﺮ ﺻﻔﺎت ﻣﻮرﻓﻮﻟﻮژﻳﻚ و ﺷﺎﺧﺺ ﻛﻠﺮوﻓﻴﻞ در ﻛﺸﺖ درون ﺷﻴﺸﻪای ﻋﻠﻒ ﭼﺎی ( . Hypericum perforatum L )

hypericum perforatum from hypericaceae family is important medicinal plant and contains active ingredients including hypericin and hyperforin. This plant has anti-bacterial, antidepressants and anti-inflammatary properties. This study was undertaken to investigation of effect of different concentrations of salicylic acid (0, 200, 400, 600 micro molar (µ.M)) and MS and B5 medium concentrations (50 % and 100%). Salicylic acid treatments were applied after 40 days. Experiment was conducted in a completely randomized design with three replications. Teen days after the treatments, morphological characteristic and leaf chlorophyll index was examined. The results indicate significant differences between the culture mediums in the shoot number and shoot length, node number in longest shoot, and chlorophyll content. Salicylic acid had significant effect on all characteristics except node number. The most shoot number was shown in B5 and ½ B5 medium supplemented with 400 µ.M of salicylic acid, in four culture media increasing salicylic acid concentration up to 400 µ.M, led to increase the chlorophyll content. Based on shoot length and node number on longest shoot, MS mediums was better than B5 mediums.