تاثیر متیل جاسمونات و محیط کشت بر رشد ریشه و گیاهچه در علف چای

Hypericum perforatum from hypericaceae has several pharmacological effects like anti-depressant and antiinflammatory.
This study aimed to evaluate the effect of methyl jasmonate with various concentration (0,
300, 600, 900 micro molar (μ.M)) and culture Media MS and B5 (50% , 100%) on Hypericum perforatum in
in-vitro culture condition. The seeds sterilized by 70% ethanol and sodium hypochlorite (50%), and then
were cultured in different culture media. Experiment was conducted in a completely randomized design with
three replications. Methyl jasmonate treatments were applied after 40 days. Fourteen days after the
treatments, root and plantlet growth and hypericin concentration were Measured. The results showed that
between culture media based on dry and wet weight of roots and plantlet there was significant difference.
Methyl jasmonate concentrations had significant effect on all of characteristcs except plantlet dry weight.
There was significant interaction between mediums and methyl jasmonate based on wet and dry
weight of root and plantlet and Hypericin concentration. Dry weight of roots in ½ B5 was lower than
others. In B5 and ½ B5, dry weight of plantlet was lower than MS and ½ MS. Our results showed that the
response of Hypericum perforatum to methyl jasmonate in MS medium is better than B5.