همسانه سازی بخشی از ژن کدکننده آنزیم کیتیناز کلاس 3 از گیاه کدو حلوایی

Chitinases act by hydrolytically cleaving the β-glycosidic linkages between GlcNAc. They are among the most important groups of pathogenesis related proteins conferring plants resistance to wide variety of fungal pathogens. These enzymes catalyze the hydrolysis of chitin, a linear homopolymer of β-1,4-linked N-acetylglucosamine residues. Among cultivated and wild cucurbits, some species have been proved to be resistant to certain fungal pathogens, which can be used as genetic resources for gene transfer and breeding of susceptible cultivated varieties. In this study degenerate primers were designed from other Cucurbitaceae family plants. Purified PCR product was cloned in pTZ57R/Z cloning vector and sequenced. The results determine the presence of class III chitinase in Cucurbita moschata using degenerate primers. Analysis of gene domains through seeking nucleotide and protein database indicated that the isolated sequence belong to class III chitinase which are commonly grouped as acidic chitinase.