ﻣﻄﺎﻟﻌﻪ ﺳﺎزﮔﺎری ﮔﻴﺎﻫﺎن درون ﺷﻴﺸﻪای اﺳﺘﻮﻳﺎ (Stevia rebaudiana)ﺑﻪ ﻣﺤﻴﻂ آزاد

Stevia (Stevia rebaudiana) is a medicinal plant belonging to the family of asteraceae. It have great economic, Because this plant have high sweeteners property. At the first step low viability and low seed germination, limit their extensive cultivation. One of the effective methods to produce this plant is in vitro technology that solve the problem of large scale production. The purpose of this investigation is the study of adaptation of in vitro plants to the free environment. For this purpose were used of six culture substrate (crop soil, sand, perlite, gravel, perlite + sand (1:1) and gravel + sand (1:1)) with three irrigation solution (drinking water, 1/2 MS and hoagland solution), That was conducted in a factorial experiment based on randomized complete design with three replications. After 4 weeks length of shoot and survival percentage were evaluated. The results showed that the maximum length of shoot and survival percentage obtained in culture substrate of perlite and gravel with hoagland nutrient solution.