ﺮرﺳﻲ ﻓﻌﺎﻟﻴﺖ ﺿﺪ ﺑﺎﻛﺘﺮﻳﺎﻳﻲ اﺳﺎﻧﺲ و ﻋﺼﺎره ﻫﺎی ﻣﺨﺘﻠﻒ Ajuga chamaecistus ﺑﻪ روش رﻗﺖ ﻣﺎﻳﻊ درﻣﻴﻜﺮوﭘﻠﻴﺖ
پذیرفته شده برای پوستر
اصل مقاله (216.3 K)
عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
ﺳﺎزﻣﺎن ﭘﮋوﻫﺶ ﻫﺎی ﻋﻠﻤﻲ و ﺻﻨﻌﺘﻲ اﻳﺮان، ﭘﮋوﻫﺸﻜﺪه ﻛﺸﺎورزی، ﺗﻬﺮان، اﻳﺮان
چکیده
در ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ، ﻓﻌﺎﻟﻴﺖ ﺿﺪ ﻣﻴﻜﺮوﺑﻲ ﺑﺎﻓﺖ ﺑﺮگ Ajuga chamaecistus ﺗﺤﺖ ﺷﺮاﻳﻂ آزﻣﺎﻳﺸﮕﺎﻫﻲ ﻣﻮرد ﺑﺮرﺳﻲ ﻗﺮار ﮔﺮﻓﺖ ﻧﻤﻮﻧﻪ ﺑﺮﮔﻲ ﺧﺸﻚ ﺷﺪه A. chamaecistus ﺟﻬﺖ ﺗﻬﻴﻪ ﻋﺼﺎره ﺑﻪ ﺗﺮﺗﻴﺐ ﺑﻮﺳﻴﻠﻪ ﺣﻼل ﻫﺎی ﻫﮕﺰان، دی ﻛﻠﺮوﻣﺘﺎن )DCM( و ﻣﺘﺎﻧﻮل اﺳﺘﺨﺮاج ﺷﺪ و اﺳﺘﺨﺮاج اﺳﺎﻧﺲ ﺑﻪ روش ﺗﻘﻄﻴﺮ ﺑﺎ ﺑﺨﺎر آب ﺑﺎ اﺳﺘﻔﺎده از دﺳﺘﮕﺎه ﻛﻠﻮﻧﺠﺮ اﻧﺠﺎم ﮔﺮدﻳﺪ. ﺳﭙﺲ ﻓﻌﺎﻟﻴﺖ ﺿﺪ ﺑﺎﻛﺘﺮﻳﺎﻳﻲ ﻋﺼﺎره ﻫﺎ و اﺳﺎﻧﺲ ﺣﺎﺻﻞ ﺑﺮ روی ﮔﻮﻧﻪ ﻫﺎی ﺑﺎﻛﺘﺮﻳﺎﻳﻲ ﮔﺮم ﻣﺜﺒﺖ )Staphylococcus aureus( و ﮔﺮم ﻣﻨﻔﻲ )Escherichia coli( ﺗﺤﺖ ﺷﺮاﻳﻂ آزﻣﺎﻳﺸﮕﺎﻫﻲ ﺑﻪ روش رﻗﺖ ﻣﺎﻳﻊ در ﻣﻴﻜﺮوﭘﻠﻴﺖ و ﺑﺎ اﺳﺘﻔﺎده از روش MTT ﺑﺮرﺳﻲ ﮔﺮدﻳﺪ. ﻓﻌﺎﻟﻴﺖ ﺿﺪ ﻣﻴﻜﺮوﺑﻲ ﺑﺮ روی ﮔﻮﻧﻪ ﻫﺎی ﺑﺎﻛﺘﺮﻳﻴﺎﻳﻲ ﺑﺮای ﻋﺼﺎره ﻫﺎی ﻣﺘﺎﻧﻮﻟﻲ و DCM ﻣﺸﺎﻫﺪه ﻧﮕﺮدﻳﺪ در ﺣﺎﻟﻲ ﺑﺮای ﻋﺼﺎره ﻫﮕﺰان ﻓﻌﺎﻟﻴﺖ ﺿﺪﻣﻴﻜﺮوﺑﻲ و ﺑﺎزدارﻧﺪﮔﻲ ﺗﻨﻬﺎ ﻋﻠﻴﻪ )Staphylococcus aureus( ﮔﺮم ﻣﺜﺒﺖ ﺑﺎ ﻣﻔﺪار ﺣﺪاﻗﻞ ﻏﻠﻈﺖ ﻛﺸﻨﺪﮔﻲ )MIC(
4mg/ml ﻣﺸﺎﻫﺪه ﺷﺪ. ﻧﺘﺎﻳﺞ ﻧﺸﺎن داد ﻛﻪ ﺗﻨﻬﺎ اﺳﺎﻧﺲ دارای ﻓﻌﺎﻟﻴﺖ ﺿﺪ ﻣﻴﻜﺮوﺑﻲ ﺑﺮ روی ﻫﺮ دو ﮔﻮﻧﻪ ﺑﺎﻛﺘﺮی ﻫﺎی ﮔﺮم ﻣﺜﺒﺖ و ﮔﺮم ﻣﻨﻔﻲ ﺑﺎ ﻣﻘﺪار V / V) ٪2/5 MIC( ﻣﻲ ﺑﺎﺷﺪ. ﻧﺘﺎﻳﺞ اراﺋﻪ ﺷﺪه در اﻳﻨﺠﺎ ﻧﺸﺎن ﻣﻲ دﻫﺪ ﻛﻪ اﺳﺎﻧﺲ A. chamaecistus دارای ﻃﻴﻒ وﺳﻴﻊ ﺗﺮی از ﻓﻌﺎﻟﻴﺖ ﻫﺎی ﺿﺪ ﺑﺎﻛﺘﺮی در ﻣﻘﺎﻳﺴﻪ ﺑﺎ ﺳﺎﻳﺮ ﻋﺼﺎره ﻫﺎی ﺣﻼل در ﺑﺮاﺑﺮ ﻫﺮ دو ﺑﺎﻛﺘﺮی ﻫﺎی ﮔﺮم ﻣﺜﺒﺖ و ﮔﺮم ﻣﻨﻔﻲ
آزﻣﺎﻳﺶ ﺷﺪه اﺳﺖ.
4mg/ml ﻣﺸﺎﻫﺪه ﺷﺪ. ﻧﺘﺎﻳﺞ ﻧﺸﺎن داد ﻛﻪ ﺗﻨﻬﺎ اﺳﺎﻧﺲ دارای ﻓﻌﺎﻟﻴﺖ ﺿﺪ ﻣﻴﻜﺮوﺑﻲ ﺑﺮ روی ﻫﺮ دو ﮔﻮﻧﻪ ﺑﺎﻛﺘﺮی ﻫﺎی ﮔﺮم ﻣﺜﺒﺖ و ﮔﺮم ﻣﻨﻔﻲ ﺑﺎ ﻣﻘﺪار V / V) ٪2/5 MIC( ﻣﻲ ﺑﺎﺷﺪ. ﻧﺘﺎﻳﺞ اراﺋﻪ ﺷﺪه در اﻳﻨﺠﺎ ﻧﺸﺎن ﻣﻲ دﻫﺪ ﻛﻪ اﺳﺎﻧﺲ A. chamaecistus دارای ﻃﻴﻒ وﺳﻴﻊ ﺗﺮی از ﻓﻌﺎﻟﻴﺖ ﻫﺎی ﺿﺪ ﺑﺎﻛﺘﺮی در ﻣﻘﺎﻳﺴﻪ ﺑﺎ ﺳﺎﻳﺮ ﻋﺼﺎره ﻫﺎی ﺣﻼل در ﺑﺮاﺑﺮ ﻫﺮ دو ﺑﺎﻛﺘﺮی ﻫﺎی ﮔﺮم ﻣﺜﺒﺖ و ﮔﺮم ﻣﻨﻔﻲ
آزﻣﺎﻳﺶ ﺷﺪه اﺳﺖ.
کلیدواژه ها
Title
The in vitro antibacterial activities of essential oil and different solvent extracts of Ajuga chamaecistus
Authors
Abstract
Abstract
In present study, in vitro antimicrobial activities of Ajuga chamaecistus leaves were investigated. The dried leaves of A. chamaecistus were sequentially extracted by hexane, dichloromethane (DCM) and methanol. The dried leaves for essential oil extraction subjected to hydrodistillation also. The extracts were then screened for in vitro antimicrobial activity against one Gram-positive (Staphylococcus aureus) and one Gram-negative (Escherichia coli) bacteria species by using two-fold serial dilution method and MTT assay. No antimicrobial activity against the bacteria was detected for DCM and methanol extracts whereas inhibitory activity was found in the hexane extract against only Gram-positive (Staphylococcus aureus) bacteria with MIC value of 4 mg/ml. It was determined that only essential oil has antimicrobial activity against both tested Gram-positive and Gram-negative bacteria species with MIC value of %2.5 (V/V). Results presented here may suggest that the essential oil of A. chamaecistus has a broader spectrum of anti-bacterial activity compare other solvent extracts against both tested Gram-positive and Gram-negative bacteria.
In present study, in vitro antimicrobial activities of Ajuga chamaecistus leaves were investigated. The dried leaves of A. chamaecistus were sequentially extracted by hexane, dichloromethane (DCM) and methanol. The dried leaves for essential oil extraction subjected to hydrodistillation also. The extracts were then screened for in vitro antimicrobial activity against one Gram-positive (Staphylococcus aureus) and one Gram-negative (Escherichia coli) bacteria species by using two-fold serial dilution method and MTT assay. No antimicrobial activity against the bacteria was detected for DCM and methanol extracts whereas inhibitory activity was found in the hexane extract against only Gram-positive (Staphylococcus aureus) bacteria with MIC value of 4 mg/ml. It was determined that only essential oil has antimicrobial activity against both tested Gram-positive and Gram-negative bacteria species with MIC value of %2.5 (V/V). Results presented here may suggest that the essential oil of A. chamaecistus has a broader spectrum of anti-bacterial activity compare other solvent extracts against both tested Gram-positive and Gram-negative bacteria.
Keywords
Ajuga chamaecistus, Antimicrobial activity, Essential oil, Micro-dilution assay, Solvent extracts