کاهش خسارات اکسیداتیو ناشی از شوری در کیلان چینی از طریق کاربرد و مداخله اسید اسکوربیک
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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1ﻛﺎرﺷﻨﺎس ارﺷﺪ زراﻋﺖ، داﻧﺸﮕﺎه آزاد اﺳﻼﻣﻲ واﺣﺪ ﺷﻬﺮ ﻗﺪس
2پزوﻫﺸﮕﺮ، داﻧﺸﻜﺪه ﻛﺸﺎورزی، داﻧﺸﮕﺎه ﭘﻮﺗﺮا ﻣﺎﻟﺰی
چکیده
ﺑﻤﻨﻈﻮر ارزﻳﺎﺑﻲ ﺗﺎﺛﻴﺮ ﻣﺜﺒﺖ ﺑﺮﮔﭙﺎﺷﻲ اﺳﻴﺪ اﺳﻜﻮرﺑﻴﻚ ﺑﺮ ﺗﻮاﻧﺎﻳﻲ ﺳﺎزﮔﺎری ﮔﻴﺎه ﻛﻴﻼن ﭼﻴﻨﻲ Brassica alboglabra L.)( ﺑﻪ ﺷﻮری، ﮔﻴﺎﻫﭽﻪ ﻫﺎی ﺟﻮان )21 روز ﭘﺲ از ﺟﻮاﻧﻪ زﻧﻲ( ﺑﺎ اﺳﺘﻔﺎده از اﺳﻴﺪ اﺳﻜﻮرﺑﻴﻚ )0 و 10 ﻣﻴﻠﻲ ﻣﻮﻻر( ﺑﺮای ﻣﺪت 16 روز ﺗﻴﻤﺎر ﺷﺪﻧﺪ. ﻓﻌﺎﻟﻴﺖ آﻧﺘﻲ اﻛﺴﻴﺪاﻧﻬﺎی آﻧﺰﻳﻤﻲ ﺷﺎﻣﻞ ﻛﺎﺗﺎز (CAT)، ﭘﺮاﻛﺴﻴﺪاز (POX) و اﺳﻜﻮرﺑﺎت ﭘﺮاﻛﺴﻴﺪاز (APX) ﺑﻪ ﻫﻤﺮاه ﺗﻐﻴﻴﺮات ﻣﺤﺘﻮی ﭘﺮوﺗﺌﻴﻦ، ﻛﻠﺮوﻓﻴﻞ و ﻣﻴﺰان ﭘﺮ اﻛﺴﻴﺪاﺳﻴﻮن ﭼﺮﺑﻲ در ﻧﻤﻮﻧﻪ ﻫﺎی ﺑﺮﮔﻲ ﺳﻨﺠﻴﺪه ﺷﺪﻧﺪ. ﺗﻐﻴﻴﺮات واﺿﺢ ﻧﺎﺷﻲ از ﺷﻮری ﺑﺮ ﻓﻌﺎﻟﻴﺖ آﻧﺘﻲ اﻛﺴﻴﺪاﻧﻬﺎی آﻧﺰﻳﻤﻲ، ﭘﺮاﻛﺴﻴﺪاﺳﻴﻮن ﭼﺮﺑﻲ و ﻣﺤﺘﻮی ﭘﺮوﺗﺌﻴﻦ و ﻛﻠﺮوﻓﻴﻞ ﻣﺸﺎﻫﺪه ﮔﺮدﻳﺪ. ﻛﺎرﺑﺮد اﺳﻴﺪ اﺳﻜﻮرﺑﻴﻚ ﻣﻨﺠﺮ ﺑﻪ اﻓﺰاﻳﺶ ﻓﻌﺎﻟﻴﺖ CAT و POX، ﻛﺎﻫﺶ ﻓﻌﺎﻟﻴﺖ APX و ﭘﺮ اﻛﺴﻴﺪاﺳﻴﻮن ﭼﺮﺑﻲ و ﻧﻴﺰ اﻓﺰاﻳﺶ ﻣﺤﺘﻮی ﭘﺮوﺗﺌﻴﻦ و ﻛﻠﺮوﻓﻴﻞ ﮔﺮدﻳﺪ. ﻧﺘﺎﻳﺞ اﻳﻦ ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎن داد ﻛﻪ ﻛﺎرﺑﺮد اﺳﻴﺪ اﺳﻜﻮرﺑﻴﻚ ﺑﺎ ﺗﻨﻈﻴﻢ ﻣﻜﺎﻧﻴﺰم دﻓﺎﻋﻲ و ﻛﺎﻫﺶ ﺧﺴﺎرات ﻛﺸﻨﺪه اﻛﺴﻴﺪاﺗﻴﻮ ﻣﻴﺘﻮاﻧﺪ ﺗﺤﻤﻞ ﮔﻴﺎه را ارﺗﻘﺎ دﻫﺪ.
کلیدواژه ها
Title
Alleviation of salt induced oxidative injury of Chinese kale (Brassica alboglabra L.) through ascorbic acid intervention
Authors
Abstract
In order to assess the supportive effect of foliar application with ascorbic acid on the survival capability of Chinese kale (Brassica alboglabra L.) plants facing salinity, 21-day-old salinized
seedlings were exogenously treated with ascorbic acid (0 and 10 mM) for 16 days. The activity of
antioxidant enzymes including, catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APX) along with the changes in the contents of protein, chlorophyll and size of lipid peroxidation were estimated in the leaf samples. Obvious salt-induced changes in the activity of antioxidant enzymes, lipid peroxidation, and contents of protein and chlorophyll were observed. Exogenous ascorbic acid resulted in a higher CAT and POX activity, lower APX activity and lipid peroxidation as well as higher protein and chlorophyll contents. The results of this study showed that the exogenous ascorbic acid with regulation in the defense mechanism and alleviation of the detrimental oxidative-injuries can promote plant tolerance.
seedlings were exogenously treated with ascorbic acid (0 and 10 mM) for 16 days. The activity of
antioxidant enzymes including, catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APX) along with the changes in the contents of protein, chlorophyll and size of lipid peroxidation were estimated in the leaf samples. Obvious salt-induced changes in the activity of antioxidant enzymes, lipid peroxidation, and contents of protein and chlorophyll were observed. Exogenous ascorbic acid resulted in a higher CAT and POX activity, lower APX activity and lipid peroxidation as well as higher protein and chlorophyll contents. The results of this study showed that the exogenous ascorbic acid with regulation in the defense mechanism and alleviation of the detrimental oxidative-injuries can promote plant tolerance.
Keywords
salinity, oxidative stress, Ascorbic acid, enzymatic defense