تنوع اللی ژن‌های زیر‌واحد گلوتنین با وزن مولکولی پایین در ژنوتیپ‌های بومی گندم نان بهاره ایرانی

In wheat breeding programs, quality bread baking is one of the most important priorities. Therefore, production of high yield varieties with improved bread baking is important. In the present study, allelic diversity of LMW-GS genes was analyzed in 154 Iranian spring wheat landraces and Chinese spring cultivar using group-specific primers. For Glu-A3, Glu-B3 and Glu-D3 loci, in total, 12, 2 and 9 alleles, respectively were amplified. Using two primer pairs Glu3A.2 and Glu3A.3, gene sub-groups coding proteins with the N-terminal sequences of MDTSCIP- and METSCIP- were amplified in Glu-A3 locus. The fragment of 700-bp length with 45.3% and fragment with size of 742-bp with 0.2% showed maximum and minimum frequency in this locus. In Glu-B3 locus, using Glu3B.2 primer pair designed based gene sub-groups coding proteins with the N-terminal sequence of METSHIPG-, two 440 and 421-bp fragments with frequency of 73.2 and 26.8%, respectively were amplified. The Glu3D.2, Glu3D.3 and Glu3D.4 primer pairs designed based on gene sub-groups coding proteins with the N-terminal sequences of METSRV-, METCIP-and METSCIP- were used to amplify Glu-D3 locus. In total, 10 alleles were amplified and 700-bp allele with 34% and 589-bp allele with 0.7% showed maximum and minimum allele frequency, respectively.