ارزیابی‌مقاومت بیوتیپ مقاوم علف‌هرز خردل‌وحشی (Sinapis arvensis L.) به علف‌کش تری‌بنورون‌متیل با استفاده از روش زیست‌سنجی بذر در پتری‌دیش

In order to evaluate the resistance of wild mustard (Sinapis arvensis L.) to tribenuron-methyl herbicide, seed bioassay experiments were performed at research agronomy laboratory of Gorgan university of agricultural science and natural resource, Iran in 2013. The type of design was completely randomized design, with four replications per treatment. Treatments included twenty one Sinapis arvensis biotypes were collected from 3 cities of Golestan province including Gorgan, Aliabad, Agh'ghala, along with a susceptible biotype, from regions with no chemical control record. The study was carried out in two steps including screening wild mustard biotypes with discriminating dose in greenhouse and seed bioassay experiment in Petri dish. In the screening test with discriminating dose, 4 biotypes were determined as a suspected resistant including, Al S3, Ag S1, G S2 and 108-1S. For this 4 biotypes root length (% of control) in petri dish, 7 days after herbicide application was used as a plant growth index. According to the seed bioassay test, the effective dose values of Al S3, Ag S1, G S2 and 108-1S and susceptible biotypes were 26.6, 43.01, 6.17, 10.51and 3.37