الگوی نواری دو ایزوزیم سوپراکسید دیسموتاز در گیاهچه ژنوتیپهای لوبیا تحت تنش خشکی
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اصل مقاله (158.6 K)
عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1کارشناسی ارشد بیوتکنولوژی کشاورزی
2گروه به نژادی و بیوتکنولوژی گیاهی
چکیده
آﻧﺰﻳﻢﻫﺎی آﻧﺘﻲاﻛﺴﻴﺪان ﻧﻘﺶ ﻣﻬﻤﻲ در ﺣﻔﻆ ﺳﻠﻮل در ﺑﺮاﺑﺮ ﺻﺪﻣﺎت ﮔﻮﻧﻪﻫﺎی ﻓﻌﺎل اﻛﺴﻴﮋن اﻳﺠﺎد ﺷﺪه در ﺷﺮاﻳﻂ ﺗﻨﺶ ﺧﺸﻜﻲ دارﻧﺪ. در اﻳﻦ ﭘﮋوﻫﺶ اﺛﺮ ﺗﻨﺶ ﺧﺸﻜﻲ ﻧﺎﺷﻲ از ﭘﻠﻲاﺗﻴﻠﻦﮔﻠﻴﻜﻮل ﺑﺮ ﻓﻌﺎﻟﻴﺖ اﻳﺰوزﻳﻢﻫﺎی آﻧﺘﻲاﻛﺴﻴﺪان ﺳﻮﭘﺮاﻛﺴﻴﺪ دﻳﺴﻤﻮﺗﺎز )(SOD در ﮔﻴﺎﻫﭽﻪﻫﺎی 60 ژﻧﻮﺗﻴﭗ ﻟﻮﺑﻴﺎ در ﺳﻪ رﻧﮓ ﺳﻔﻴﺪ، ﻗﺮﻣﺰ و ﭼﻴﺘﻲ( در ﻗﺎﻟﺐ ﻃﺮح ﻛﺎﻣﻼً ﺗﺼﺎدﻓﻲ در ﺳﻪ ﺗﻜﺮار در ﻣﻘﺎﻳﺴﻪ ﺑﺎ ﺷﺮاﻳﻂ ﻋﺎدی ﻣﻮرد ارزﻳﺎﺑﻲ ﻗﺮار ﮔﺮﻓﺖ. آزﻣﺎﻳﺶ ﺑﻪ ﺻﻮرت ﻓﺎﻛﺘﻮرﻳﻞ ﺑﺎ ﻃﺮح ﭘﺎﻳﻪ ﻛﺎﻣﻼً ﺗﺼﺎدﻓﻲ اﺟﺮا ﺷﺪ. ﻳﻜﻲ از ﻓﺎﻛﺘﻮرﻫﺎ ﺷﺮاﻳﻂ آﺑﻴﺎری ﺑﺎ ﻣﺤﻠﻮل ﭘﻠﻲ اﺗﻴﻠﻦ ﮔﻠﻴﻜﻮل (PEG ) ل3 -بار و آب معمولی و فاکتور دیگر سه رنگ لوبیا در نظر گرفته شد. تجزیه الکتروفورزی با استفاده از ژل ﭘﻠﻲآﻛﺮﻳﻞآﻣﻴﺪ اﻓﻘﻲ 8 درﺻﺪ اﻧﺠﺎم ﮔﺮدﻳﺪ و اﻳﺰوزﻳﻢﻫﺎی SOD در ﻳﻜﻲ از ﻻﻳﻪﻫﺎی ژل رﻧﮓآﻣﻴﺰی ﺷﺪﻧﺪ. ﺑﺮای ﻫﺮ ﻧﻮار در روی ژل ﻣﻴﺰان ﻣﺴﺎﺣﺖ ﺷﺪت" ﺑﻪ ﻋﻨﻮان ﻓﻌﺎﻟﻴﺖ اﻳﺰوزﻳﻤﻲ ﺑﺎ ﻧﺮماﻓﺰار MCID ارزﻳﺎﺑﻲ و ﻳﺎدداﺷﺖ ﮔﺮدﻳﺪ. ﭼﻬﺎر اﻳﺰوزﻳﻢ ﺑﺮای SODﺷﻨﺎﺳﺎﻳﻲ ﺷﺪ. ﻓﻌﺎﻟﻴﺖ دو ﺗﺎ از ﭼﻬﺎر اﻳﺰوزﻳﻢ ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻗﺮار ﮔﺮﻓﺖ و ﻣﺸﺨﺺ ﺷﺪ ﻓﻌﺎﻟﻴﺖ آﻧﻬﺎ ﺗﺤﺖ ﺗﺄﺛﻴﺮ ژﻧﻮﺗﻴﭗﻫﺎ و ﺳﻄﻮحﺧﺸﻜﻲ ﻗﺮار دارﻧﺪ. ﺧﺸﻜﻲ ﻣﻮﺟﺐ اﻓﺰاﻳﺶ ﻓﻌﺎﻟﻴﺖ دو اﻳﺰوزﻳﻢ1SOD و SOD2 گردید. براساس نتایج مقایسه میانگین ترکیبات تیمارهای ژنوتیپ و تنش خشکی، بیشترین فعالیت آنزیمی ایزوزیمهای تحت تنش خشکی به ژنوتیپ 36 و در شرایط عادی به ژنوتیپ 3 تعلق داشت.
کلیدواژه ها
Title
Superoxide dismutase profiling in common bean seedling under drought stress
Authors
Abstract
Antioxidant enzymes play major role in cell protection against damages caused by active oxygen species at
drought stress condition. In this study drought stress effects caused by polyethylene glycol on superoxide
dismutase antioxidant isozymes activities on 60 common bean genotypes seedlings in three colors (whitered-
pinto) were evaluated based on completely randomized design in three replication under normal
irrigation and PEG mediated drought stress conditions. One of the factors was irrigation condition with PEG
(-3 bar) and the other factor was color of bean genotypes. Electrophoresis analysis was done by 8 percent
poly acrylamide gel and SOD isozymes were stained in one of the horizontal gel layers. For each SOD band
on the gel the amount of “rate distance” was evaluated and recorded as isozymes activity by MCID
software. Four isozymes for SOD were recognized. Isozymes activities were affected by genotypes and
drought levels. Drought stress increased the activity of two isozymes SOD1 and SOD2 According to results of comparing the average amounts of genotype varieties and drought activity, maximum
densitometeric activity of SOD1 and SOD2 isozymes under drought stress was related to genotype 36 and
under common condition was related to genotype 3.
drought stress condition. In this study drought stress effects caused by polyethylene glycol on superoxide
dismutase antioxidant isozymes activities on 60 common bean genotypes seedlings in three colors (whitered-
pinto) were evaluated based on completely randomized design in three replication under normal
irrigation and PEG mediated drought stress conditions. One of the factors was irrigation condition with PEG
(-3 bar) and the other factor was color of bean genotypes. Electrophoresis analysis was done by 8 percent
poly acrylamide gel and SOD isozymes were stained in one of the horizontal gel layers. For each SOD band
on the gel the amount of “rate distance” was evaluated and recorded as isozymes activity by MCID
software. Four isozymes for SOD were recognized. Isozymes activities were affected by genotypes and
drought levels. Drought stress increased the activity of two isozymes SOD1 and SOD2 According to results of comparing the average amounts of genotype varieties and drought activity, maximum
densitometeric activity of SOD1 and SOD2 isozymes under drought stress was related to genotype 36 and
under common condition was related to genotype 3.