ﻣﻜﺎن ﻳﺎﺑﻲQTL های کنترل کننده صفات مرتبط با تحمل به خشکی گندم نان (تلاقی روشن * سبلان )
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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1دانشجوی کارشناسی ارشد اصلاح نباتات دانشگاه شهید باهنر کرمان
2دانشیاراصلاح نباتات،دانشکده کشاورزی،دانشگاه شهید باهنرکرمان
3استاد یار پژوهشکده بیوتکنولوژی کشاورزی ایران، کرج، ایران
چکیده
ﺑﻪ ﻣﻨﻈﻮر ﻣﻜﺎن ﻳﺎﺑﻲQTLﻫﺎی ﻛﻨﺘﺮل ﻛﻨﻨﺪه ﺻﻔﺎت زراﻋﻲ ﻣﺮﺗﺒﻂ ﺑﺎ ﺗﺤﻤﻞ ﺑﻪ ﺧﺸﻜﻲ ﮔﻨﺪم ﻧﺎن،129ﻻﻳﻦ ﻧﻮﺗﺮﻛﻴﺐ ﺧﺎﻟﺺ ﺣﺎﺻﻞ ازﺗﻼﻗﻲ روﺷﻦ × ﺳﺒﻼن ﺑﻪ ﻫﻤﺮاه ﺳﻪ ﺷﺎﻫﺪ روﺷﻦ و ﺳﺒﻼن)واﻟﺪﻳﻦ( و ﻣﻬﺪوی( درﻗﺎﻟﺐ ﻃﺮح آﮔﻤﻨﺖ در ﻣﺰرﻋﻪ داﻧﺸﻜﺪه ﻛﺸﺎورزی ﺑﺎﻫﻨﺮ در ﺳﺎل زراﻋﻲ 92- 91 ﻣﻮردآزﻣﻮن ﻗﺮارﮔﺮﻓﺘﻨﺪ.ﻣﻄﺎﻟﻌﻪ ﻣﻮﻟﻜﻮﻟﻲ ﺑﻪ ﻣﻨﻈﻮرﺷﻨﺎﺳﺎﻳﻲ ﻫﺎ،ﺑﺮاﺳﺎس اﻃﻼﻋﺎت580 ﻧﺸﺎﻧﮕﺮ دارت،ﺑﺮروی ﺟﻤﻌﻴﺖ اﻳﻨﺒﺮدﻻﻳﻦ ﺣﺎﺻﻞ ازﺗﻼﻗﻲ روﺷﻦ وﺳﺒﻼن اﻧﺠﺎم ﺷﺪ و ﺑﺮاﺳﺎس اﻃﻼﻋﺎت ژﻧﺘﻴﻜﻲ ﮔﺰﻳﻨﺶ ﺷﺪه ﻧﻘﺸﻪ ﭘﻴﻮﺳﺘﮕﻲ ﺗﻬﻴﻪ ﺷﺪ. ﻧﺸﺎﻧﮕﺮﻫﺎ، 16ﮔﺮوه ﻟﻴﻨﻜﺎژی راﺗﺸﻜﻴﻞ دادﻧﺪﻛﻪ 652.486 ﺳﺎﻧﺘﻲ ﻣﻮرﮔﺎن از ﻛﻞ ژﻧﻮم ﺗﻮﺳﻂ ﻧﺸﺎﻧﮕﺮ ﻫﺎ ﭘﻮﺷﺶ داده شد.ﺑﺎروش ﻣﻜﺎن ﻳﺎﺑﻲ ﻓﺎﺻﻠﻬﺎی ﻣﺮﻛﺐ در ﻣﺠﻤﻮعQTL 23 دارای LOD بالای 2,5 شناسایی شد،که کنترل 6صفت مورد بررسی رابرعهده داشتند. 6QTL برای صفت تعداد پنچه کل، 7QTL برای تعداد بذر در بوته ،یک برای وزن هزار دانه ، 3 QTL برای شاخص برداشت یافت شد QTLمربوط به تعداد پنجه کل بیشترینLOD= 4.9 را داشت که 16 درصد از تغییرات فنوتیپی را توجیه میکند. اطلاعات بدست آمده ازQTL های شناسایی شده رهیافت مناسبی برای بهبود سازوکار تحمل به خشکی و در نتیجه بهبود عملکرد در شرایط تنش خشکی خواهد بود.
کلیدواژه ها
Title
Locating of QTLs Controlling Traits Related to Drought Tolerancein Wheat(Crosses between Roshan and Sabalan)
Authors
Abstract
Abstract
In order to detect controlling QTLs of important agronomic traits in bread wheat,129 different recombinant inbred
lines derived from crosses between Roshan&Sabalan with three check cultivars ( Roshan, Sabalan(as the parent) and
Mahdavi) were investigated based on Augmented design, in research field of Shahid Bahonar university of Kerman on
growing season of 2012-2013. Molecular study of different lines was done using 580DArT markers on recombinant
inbred line population, based on markers 16 linkage groups were obtained 652.486 CentiMorgan of total length of
genome. linkage map was prepared based on the selected genetic information. Based on Composite interval mapping
method, 23 QTLs with LOD higher than 2.5 were identified, which were belonged to 6 traits. 6 QTLs for No. of total
tillers,7QTLs for number of seeds per plant, 1QTL for grain number per main spike, 2QTLs for grain weight per main
spike,1 QTL for thousand seed weight and3QTLs for HI were detected. QTL which related to total tillers had the
highest LOD = 4.7 that justified 16% of phenotypic variation. Data from the identified QTLs will be perfect approach
for improving drought tolerance mechanisms and thus improve yield under dro
In order to detect controlling QTLs of important agronomic traits in bread wheat,129 different recombinant inbred
lines derived from crosses between Roshan&Sabalan with three check cultivars ( Roshan, Sabalan(as the parent) and
Mahdavi) were investigated based on Augmented design, in research field of Shahid Bahonar university of Kerman on
growing season of 2012-2013. Molecular study of different lines was done using 580DArT markers on recombinant
inbred line population, based on markers 16 linkage groups were obtained 652.486 CentiMorgan of total length of
genome. linkage map was prepared based on the selected genetic information. Based on Composite interval mapping
method, 23 QTLs with LOD higher than 2.5 were identified, which were belonged to 6 traits. 6 QTLs for No. of total
tillers,7QTLs for number of seeds per plant, 1QTL for grain number per main spike, 2QTLs for grain weight per main
spike,1 QTL for thousand seed weight and3QTLs for HI were detected. QTL which related to total tillers had the
highest LOD = 4.7 that justified 16% of phenotypic variation. Data from the identified QTLs will be perfect approach
for improving drought tolerance mechanisms and thus improve yield under dro
Keywords
QTL, Diversity Arrays Technology of DNA, breed wheat