تأثیرغلظتهای مختلف سینامیکاسیدو زمانهای مختلف تلقیح بر القای ریشه مویین به واسطه آگروباکتریوم رایزوژنزدر گیاه دارویی کتان سفی
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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1دانشجوی کارشناسی ارشد، گروه اصلاح و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه ارومیه
2استادیار، گروه اصلاح و بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه ارومیه
چکیده
ﻛﺸﺖ ﺑﺎﻓﺖ ﮔﻴﺎﻫﺎن داروﻳﻲ ﺑﻪ ﻋﻨـﻮان ﻳـﻚ راه ﺣـﻞ ﺟﻬـﺖ ﺗﻮﻟﻴـ ﺪ ﻣﺘﺎﺑﻮﻟﻴـ ﺖﻫـﺎ ی ﺛﺎﻧﻮﻳـ ﻪ ﺑـﺎ ارزش ﻣﻌﺮﻓـ ﻲ ﺷـﺪه اﺳـﺖ و اﺧﻴـ ﺮاً ﻛﺸﺖ رﻳﺸﻪﻫﺎی ﻣﻮﻳﻴﻦ ﺑﻪ ﻋﻨﻮان ﻳﻚ ﻣﻨﺒﻊ ﭘﺎﻳﺪار ﺑﺮای ﺗﻮﻟﻴـ ﺪ ﻣﺘﺎﺑﻮﻟﻴـ ﺖﻫـﺎ ﭘﻴﺸـﻨﻬﺎد ﺷـﺪه اﺳـ ﺖ. رﻳﺸـﻪ ﻫـﺎ ی ﻣـﻮ ﻳﻴﻦ ﻛـ ﻪ ﺑـﺎ اﺳـﺘﻔﺎده از اﮔﺮوﺑﺎﻛﺘﺮﻳﻮم راﻳﺰوژﻧﺰ ﻳـﻚ ﻧـﻮع ﺑـﺎ ﻛﺘﺮی ﮔـﺮم ﻣﻨﻔـ ﻲ ﺧـﺎ ﻛﺰی( ﺗﻮﻟﻴـ ﺪ ﻣـ ﻲﺷـﻮﻧﺪ دارای رﺷـﺪ ﺳـﺮ ﻳﻊ ﺑـﻮده و ﺑﺰرﮔﺘـﺮ ﻳﻦ ﻣﺰﻳـ ﺖ ﻛﺸﺖ رﻳﺸﻪﻫﺎی ﻣﻮﻳﻴﻦ در ﻣﻘﺎﻳﺴـﻪ ﺑـﺎ ﮔﻴﺎﻫـﺎن ﻣﺮﺑـﻮط ، ﺗـﻮان ﺑـﺎﻻ ﻳﻲ در ﺗﻮﻟﻴـ ﺪ ﻣﺘﺎﺑﻮﻟﻴـ ﺖﻫـﺎ ی ﺛﺎﻧﻮﻳـ ﻪ دارﻧـﺪ. ﻫـﺪف از اﻳـﻦ ﺗﺤﻘﻴـﻖ ﺑﺮرﺳﻲ اﺛﺮ ﻏﻠﻈـﺖ ﻫـﺎی μM 150 و 200 ﺳـﻴﻨﺎﻣﻴﻚ اﺳـﻴﺪ و ﻣـﺪت زﻣـﺎن ﻫـﺎی5 و 3 دﻗﻴﻘـﻪ ﺗﻠﻘـﻴﺢ ﺑـﺮ ﺗﻮﻟﻴـﺪ رﻳﺸـﻪ ﻫـﺎی ﻣـﻮﻳﻴﻦ ﺑـﻪ ﻋﻨﻮان ﻳﻚ ﻣﻨﺒﻊ ﺗﻮﺳﻌﻪﻳﺎﻓﺘﻪ ﺑﺮای ﺗﺮﻛﻴﺒـﺎت ﺛﺎﻧﻮﻳـﻪ در ﮔﻴـﺎه داروﻳـﻲ ﻛﺘـﺎن ﺳـﻔﻴﺪ ﻣـﻲ ﺑﺎﺷـﺪ آﻧـﺎﻟﻴﺰ آﻣـﺎری ﺗﻌـﺪاد رﻳﺸـﻪ ﻫـﺎی اﻟﻘـﺎﻳﻲ اﻳــﻦ آزﻣــﺎﻳﺶ درﻗﺎﻟــﺐ ﻃــﺮح ﻛــﺎﻣﻼ ﺗﺼــﺎدﻓﻲ ﺑــﺎ 3 ﺗﻜــﺮار اﻧﺠــﺎم ﺷــﺪ و ﺑــﺮای ﺗﺄﻳﻴــﺪ درج ﻗﻄﻌــﻪ T-DNA در ژﻧــﻮم رﻳﺸــﻪﻫــﺎی ﻣــﻮﻳﻴﻦ از واﻛــﻨﺶ زﻧﺠﻴــﺮه ای ﭘﻠﻴﻤــﺮاز (PCR) ﺑــﺎ اﺳــﺘﻔﺎده از آﻏــﺎزﮔﺮ اﺧﺘﺼﺎﺻــﻲ ﺑــﺮای ﺗﻜﺜﻴــﺮ ﻫﻤﺰﻣــﺎن ﺑﺨــﺶ ﻫــﺎﻳﻲ از ژن ﻫﺎی rolA و rolB آﻧـﺎﻟﻴﺰ ﻣﻠﻜـﻮﻟﻲ اﻧﺠـﺎم ﮔﺮﻓـﺖ ﻧﺘـﺎﻳﺞ ﺣﺎﺻـﻞ از آﻧـﺎﻟﻴﺰ آﻣـﺎری اﺧـﺘﻼف ﻣﻌﻨـﻲ دار )0/01˂P ( ﺑـﻴﻦ زﻣـﺎن ﻫـﺎی ﻣﺨﺘﻠﻒ ﺗﻠﻘﻴﺢ را ﻧﺸﺎن داد. ﺑﻴﺸـﺘﺮﻳﻦ ﺗﻌـﺪاد رﻳﺸـﻪ )7-6 رﻳﺸـﻪ در ﻫـﺮ رﻳﺰﻧﻤﻮﻧـﻪ( ﻣﺮﺑـﻮط ﺑـﻪ ﻣـﺪت زﻣـﺎن ﺗﻠﻘـﻴﺢ3 دﻗﻴﻘـﻪ ﺑـﻮده و اﺧﺘﻼف ﻣﻌﻨﻲداری ﺑﻴﻦ ﻏﻠﻄﺖﻫﺎی ﻣﺨﺘﻠﻒ ﺳﻴﻨﺎﻣﻴﻚاﺳﻴﺪ ﻣﺸﺎﻫﺪه ﻧﺸﺪ.
کلیدواژه ها
Title
Effect of cinamic acid and and time of insemination on efficiency of Agrobacterium rhizogenes mediated hairy root induction in the medicianal plant Linum album
Authors
Abstract
Tissue culture of medicinal plants as a valuable solution for the production of secondary metabolites have been introduced recently as a source of stable hairy root cultures for the production of metabolites have been proposed. The hairy roots using Agrobacterium rhizogenes (a gram-negative soil bacteria) to produce fast-growing and biggest advantage of hairy root cultures in comparison with related species, can have a high production of secondary metabolites. The purpose of this study was to investigate the effect of concentration μM150 and 200 Cinamic acid 5 and 3 min inoculation time and acid production of hairy roots as a source of
The white canvas is a medicinal plant secondary compounds. Statistical analysis of the test frame for root
induction completely randomized design with three replications was used to confirm insertion of T-DNA
fragment in the genome of hairy root polymerase chain reaction (PCR) using specific primers for
simultaneous amplification part of the rolA and rolB gene molecular analysis was performed. The results of
the statistical analysis, significant differences - guard (P ˂0.01) between the time of inoculation
demonstrated. The highest number of roots (6 -7 roots per explant) relating to insemination time was 3 min
and the mean difference between the concentrations of Cinamic acid was no different.
The white canvas is a medicinal plant secondary compounds. Statistical analysis of the test frame for root
induction completely randomized design with three replications was used to confirm insertion of T-DNA
fragment in the genome of hairy root polymerase chain reaction (PCR) using specific primers for
simultaneous amplification part of the rolA and rolB gene molecular analysis was performed. The results of
the statistical analysis, significant differences - guard (P ˂0.01) between the time of inoculation
demonstrated. The highest number of roots (6 -7 roots per explant) relating to insemination time was 3 min
and the mean difference between the concentrations of Cinamic acid was no different.
Keywords
Agrobacterium rhizogenes, Cinamic acid, hairy root, Linum album