بررسی تغییرات میزان پرولین و پروتئین تحت شرایط تنش شوری در ژنوتیپ گندم طبسی و لاینهای موتانت
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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﻛﺸﺎورزی، ﮔﺮوه اﺻﻼح ﻧﺒﺎﺗﺎت و ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی، داﻧﺸﮕﺎه ﻋﻠﻮم ﻛﺸﺎورزی و ﻣﻨﺎﺑﻊ ﻃﺒﻴﻌﻲ ﮔﺮﮔﺎن
2داﻧﺸﻴﺎر ﮔﺮوه اﺻﻼح ﻧﺒﺎﺗﺎت و ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی، داﻧﺸﮕﺎه ﻋﻠﻮم ﻛﺸﺎورزی و ﻣﻨﺎﺑﻊ ﻃﺒﻴﻌﻲ ﮔﺮﮔان
3اﺳﺘﺎدﻳﺎر ﮔﺮوه اﺻﻼح ﻧﺒﺎﺗﺎت و ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی، داﻧﺸﮕﺎه ﻋﻠﻮم ﻛﺸﺎورزی و ﻣﻨﺎﺑﻊ ﻃﺒﻴﻌﻲ ﮔﺮگان
چکیده
ﮔﻨﺪم ﻣﻬﻢﺗﺮﻳﻦ ﻣﺤﺼﻮل ﻛﺸﺎورزی ﺟﻬﺎن ﺑﻪ ﺷﻤﺎر ﻣﻲرود و ﺑﺎ ﺗﻮﻟﻴﺪ ﺳﺎﻻﻧﻪ ﺣﺪود 540 ﻣﻴﻠﻴﻮن ﺗﻦ، ﻣﻘﺎم اول را در ﺑﻴﻦ ﻣﺤﺼﻮﻻت زراﻋﻲ ﺑﻪ ﺧﻮد اﺧﺘﺼﺎص داده اﺳﺖ. ﺷﻮری زﻳﺎد ﺧﺎک از ﺟﻤﻠﻪ ﻋﻮاﻣﻞ ﻣﺤﺪود ﻛﻨﻨﺪه ﻋﻤﻠﻜﺮد ﻣﺤﺼﻮﻻت در ﺳﺮﺗﺎﺳﺮ ﺟﻬﺎن ﺑﻪ ﺷﻤﺎر ﻣﻲرود ﻛﻪ اﻳﻦ ﻣﺴﺌﻠﻪ ﺑﻪ ﺧﺼﻮص در ﻣﻨﺎﻃﻖ ﺧﺸﻚ و ﻧﻴﻤﻪ ﺧﺸﻚ ﺑﻪ ﻋﻨﻮان ﻳﻜﻲ از اﺳﺎﺳﻲﺗﺮﻳﻦ ﻣﺸﻜﻼت ﺑﺨﺶ ﻛﺸﺎورزی اﺳﺖ. اﻳﻦ آزﻣﺎﻳﺶ ﺑﺎ ﻫﺪف ﺑﺮرﺳﻲ ﭘﺎﺳﺦ ﻣﻜﺎﻧﻴﺴﻢﻫﺎی ﺑﻴﻮﺷﻴﻤﻴﺎﻳﻲ ﺗﺤﻤﻞ ﺑﻪ ﺗﻨﺶ ﺷﻮری در ﮔﻨﺪم ﻧﺎن1 اﻧﺠﺎم ﺷﺪ. ﺑﺮای اﻳﻦ ﻣﻨﻈﻮر، ﺗﻐﻴﻴﺮات ﭘﺮوﺗﺌﻴﻦ و ﭘﺮوﻟﻴﻦ ﺑﺮگ ﮔﻨﺪم ، رﻗﻢ ﻃﺒﺴﻲ )واﻟﺪ( و ﻻﻳﻦﻫﺎی ﻣﻮﺗﺎﻧﺖ T-65-7-1 و T-67-60 آزﻣﺎﻳﺸﻲ در ﻣﺤﻴﻂ ﻣﺎﻳﻊ ﻳﻮﺷﻴﺪا، ﺑﻪ روش ﻫﻴﺪروﭘﻮﻧﻴﻚ اﺟﺮا ﺷﺪ. آزﻣﺎﻳﺶ ﺑﻪ ﺻﻮرت ﻓﺎﻛﺘﻮرﻳﻞ در ﻗﺎﻟﺐ ﻃﺮح ﻛﺎﻣﻼً ﺗﺼﺎدﻓﻲ ﺑﺎ ﺳﻪ ﺗﻜﺮار ﺗﺤﺖ ﺗﻨﺶ ﺷﻮری) 6 دﺳﻲ زﻳﻤﻨﺲ ﺑﺮ ﻣﺘﺮ( ﺑﻪ ﻫﻤﺮاه ﺗﻴﻤﺎر ﺷﺎﻫﺪ )ﻣﺤﻠﻮل ﻳﻮﺷﻴﺪا( اﻧﺠﺎم ﺷﺪ. ﻧﺘﺎﻳﺞ ﻧﺸﺎن داد، ﻣﻴﺰان ﺗﺠﻤﻊ ﭘﺮوﻟﻴﻦ و ﭘﺮوﺗﺌﻴﻦ در ﻻﻳﻦﻫﺎی ﻣﻮﺗﺎﻧﺖ T-65-7-1 و T-67-60 ﻧﺴﺒﺖ ﺑﻪ رﻗﻢ ﻃﺒﺴﻲ ﺗﻔﺎوت ﻣﻌﻨﻲ داری دارﻧﺪ. ﺑﻌﺒﺎرﺗﻲ ﻻﻳﻦﻫﺎی ﻣﻮﺗﺎﻧﺖ ﻃﻲ ﺗﻨﺶ ﺷﻮری ﺗﺤﻤﻞ ﻧﺴﺒﻲ ﺑﻴﺸﺘﺮی ﻧﺴﺒﺖ ﺑﻪ رﻗﻢ ﻃﺒﺴﻲ داﺷﺘﻪ اﻧﺪ.
ﻛﻠﻤﺎت ﻛﻠﻴﺪی: ﺗﻨﺶ ﺷﻮری، ﭘﺮوﻟﻴﻦ، ﭘﺮوﺗﺌﻴﻦ، ﮔﻨﺪم، ﻣﻮﺗﺎﻧﺖ
ﻛﻠﻤﺎت ﻛﻠﻴﺪی: ﺗﻨﺶ ﺷﻮری، ﭘﺮوﻟﻴﻦ، ﭘﺮوﺗﺌﻴﻦ، ﮔﻨﺪم، ﻣﻮﺗﺎﻧﺖ
کلیدواژه ها
Title
Study on protein and prolinee changes under salt stress condition in Tabasi wheat genotypes and mutant lines
Authors
Abstract
Wheat is the most important crops in the world and has the first rank between crops that have about 540 million tons annual production. Soil salinity is one of the yield determinative factors in all over the world,
especially in dry and semi-dried areas is a substantial obstacle in agriculture. This experiment aimed on
investigating the biochemical mechanisms of salt tolerance in wheat In order to determine the effects of salt stress on amount of protein and proline in leaves of two genotypes of Triticum aestivum including Tabasi and mutant lines (i.e. T-65-7-1, T-67-60), we performed this experiment in Yoshida in hydroponic method, in factorial and randomized complete design (RCD) with tree replicates. Treatment plants with 6 dS m-1 salinity and control treatment (Yoshida solution) were used.The result implied that proline and protein accumulation in T-65-7-1, T-67-60 have significant differences in contrast to Tabasi genotype. In the other word, mutant lines which have been under salt stress had more relative tolerance in contrast to Tabasi genotype.
especially in dry and semi-dried areas is a substantial obstacle in agriculture. This experiment aimed on
investigating the biochemical mechanisms of salt tolerance in wheat In order to determine the effects of salt stress on amount of protein and proline in leaves of two genotypes of Triticum aestivum including Tabasi and mutant lines (i.e. T-65-7-1, T-67-60), we performed this experiment in Yoshida in hydroponic method, in factorial and randomized complete design (RCD) with tree replicates. Treatment plants with 6 dS m-1 salinity and control treatment (Yoshida solution) were used.The result implied that proline and protein accumulation in T-65-7-1, T-67-60 have significant differences in contrast to Tabasi genotype. In the other word, mutant lines which have been under salt stress had more relative tolerance in contrast to Tabasi genotype.
Keywords
Salt stress, Proline, protein, Mutant, wheat