جداسازی ناحیه بین دو بلوک حفاظت شده موجود در توالی ژن کولینمونواکسیژناز در گندم نان

Choline monooxygenase enzyme (CMO) is a key component of glycine betaine (GB) biosynthesis pathway, playing important role in plants' biochemical adaptation to abiotic stresses. CMO genes have been identified from several plants. However, little is known about in bread wheat (Triticum aestivum). In this study, through a pair of primers designed based on two conserved motives of CMO gene, a fragment of 1000 bp were amplified in wheat that demonstrates high accordance with the expected size and sequence.