ارزیابی تنوع ژنتیکی برخی تودههای بادرنجبویه با استفاده از نشانگرهای پروتئینی

Melissa officinalis .L is a medicinal plant in the familyLamiaceae.The major purpose of present investigation is evaluation of genetic diversity based on leaf soluble protein in fifteen accessions of M. officinalis, which were collected from Iran and other region. The seeds after germination into the growth chamber were transferred to pots in green house. The leaf proteins were extracted and the proteins were separated using SDS-PAGE technique. The results of SDS-PAGE gels showed sixteen protein bands. The most of bonds existed in Hir, Damash, Shiraz accessionswith fourteen bonds. The less of bonds existed in the seeds of Esfahan Pakan Company with nine bonds. The cluster analysis of the 15 accessions using UPGMA-Ward method based on protein bands produced three clusters First group were included Germany, Japan and Iran (Hamedan, Damash, Harif and Roodbar accessions), second group were included Italy, Hungary and Iran (Hir, Shiraz, Malard, Karaj and town of Karaj village accessions), and finally, thirdgroup were contained Esfahan and Esfahan Pakan Company accessions.
The result of present study describes that protein markers indicated genetic diversity and they can use for management of germplasm and protection of successive stock in breeding programmes.