بررسی ژن‌‌ کاندید پیروفسفاتاز آرابیدوپسیس با استفاده از روش تیلینگ

TILLING (Targeting Induced Local Lesions IN Genomes) is a powerful reverse genetic technology that employs heteroduplex analysis to detect point mutations in the gene(s) of interest. Use of tilling method has increased greatly in recent years because of the possibility to rapid screening of allelic series of induced point mutation in chemically mutagenized populations. Tilling method was employed in this research to study the function of pyrophosphatase candidate gene in Arabidopsis. 24 Tilling lines with different induced point mutations in the gene of interest have examined and alteration of metabolic pattern of each line was studied using gas chromatogeraphy mass spctrophotometry. The role of candidate gene was confirmed based on the result of metabolic analysis.