ترارﻳﺨﺘﻲ ﺗﻮﺗﻮن ﺑﺎ ژنﻫﺎی رﻣﺰ ﻛﻨﻨﺪه آﻧﺘﻲﺑﺎدی ﻣﻮﻧﻮﻛﻠﻮﻧﺎل trastuzumab ﺑﻮاﺳﻄﻪ Agrobacterium tumefaciens

Cancer is one of the leading causes of death in the world, particularly in developing countries. Breast cancer is the most common cancer among women, annually more than half a million women were diagnosed with breast cancer. Trastuzumab (Herceptin) is a recombinant DNA-derived humanized antibody that is used in the treatment of metastatic breast cancer. Plant-based “molecular farming” has revealed as a promising approach with significant advantages in both cost and safety over other eukaryotic expression systems. In the present study, Agrobacterium tumefaciens strain LBA4404 harboring pBIN-Her2-HLC plasmid containing the genes encoding trastuzumab and the nptII gene (as a selectable marker gene) were used for tobacco plant transformation. Initial selection of transgenic cells was performed on medium containing kanamycin (150 mg/l). The presence of the target genes in putative transformants was confirmed by PCR analyses. The rooted transgenic plantlets were acclimatized successfully in the greenhouse, with a survival rate of 100%. They showed normal morphological characteristics similar to the maternal plant.