تاثیر کلشی سین بر روی سطح برگ استویا (Stevia rebaudiana Bertoni)

Stevia rebaudiana is one of the variety of medical and industrial plants due to the production of secondary metabolites such as stevioside has several medical and food application. this study applies the multiple treatments of various concentrations of colchicine in plants grown on MS medium with different concentrations of zero, 25, 125 and 250 milligrams per liter and different time periods (24, 48, and 72 h). Assessment plants in MS medium for leaf length, leaf width and leaf area were measured. Analysis of variation showed that the interaction of time in concentration in all traits were significant 1% probability level. Comparison of treatment means in all the studied traits showed that the superiority of colchicine concentration in 125 milligrams per liter. Leaf length, leaf width and leaf area on the third day 125 concentrations were higher than other treatments respectively 9.433 mm, 6.4 mm and 42.273 sq. mm was achieved.