مقایسه پروتئومهای برگ پرچم گندم جهت شناسایی پدیده هتروزیس در مقاومت به سپتوریا در گندم نان

Abstract
Heterosis is controlled by genes/proteins that identification of these genes in molecular approach is important issue for plant breeding projects. This study was designed to investigate heterosis effect in resistance to septoria leaf blotch disease of wheat in 3 varieties, zagros as a sensitive, N8118 as a resistant parents and their hybrid respectively. The varieties were cultured in randomized complete block design in three repeats in Agricultural Research Station of Gorgan. Inoculation of plants was done at a time. To investigation of heterosis, the proteome analysis of flag leaf tissue was used; and protein extraction was done by using TCA/Aceton method. The results of proteome analysis before inoculation showed the differential expression of proteins between wheat hybrid and their parents in 5 spots. Also the presence of one protein spot in hybrid and the absence of that spot in parents after inoculation can be candidate protein of heterosis. The purpose of this research was improvement of molecular information about heterosis and using it in plant breeding projects in the most of agronomical and physiological traits related to this phenomon, specially resistant to septoria.