بررسی بیان پروتئینهای دخیل در مقاومت به سپتوریوز برگی در برگ پرچم گندم نان

Abstrac
Nowadays, different species of Septoria destroy about two percent of wheat production all over the world annually. Accordingly, proteomics method for identifying responding proteins to septoria leaf spotch disease in zagros variety of wheat was used. Hence, seeds of Zagros variety were cultured in complete randomized design model in three replicates in Agricultural Research Station of Gorgan. The sampling was done before inoculation of flag leaf tissue, the later sampling was done after appearance of the disease on the flag leaf. Protein extraction was done via TCA/Aceton method, and extracted proteins was separated in two dimension by IPG gels. Results, outcome from gel analysis of 249 repeatable protein spot showed that at least nine protein spot have significant difference in resistance to the disease, in which 77 percent of protein expression was increased and 22 percent decreased. These results showed that investigation of proteom pattern before and after of inoculation is indicative of increase in protein spot after inoculation. Generally, it is concluded that this variety respond to this disease by expressing new proteins and increasing proteins being expressed against to septoria.