ﺑﺮرﺳﻲ ﭘﺎﻳﺪاری DNA ژﻧﻮﻣﻲ ﺗﻮدهﻫﺎی زﻳﺮه ﺳﺒﺰ در ﭘﺎﺳﺦ ﺑﻪ ﺗﻨﺶ ﻧﻴﺘﺮات ﻧﻘﺮه ﺑﺎ اﺳﺘﻔﺎده از ﻣﺎرﻛﺮ ISSR
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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ اﺻﻼح ﻧﺒﺎﺗﺎت، داﻧﺸﮕﺎه ﺷﻬﻴﺪ ﺑﺎﻫﻨﺮ ﻛﺮﻣﺎن
2اﺳﺘﺎدﻳﺎر ﺑﺨﺶ ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی، داﻧﺸﮕﺎه ﺷﻬﻴﺪ ﺑﺎﻫﻨﺮ ﻛﺮﻣﺎن
چکیده
ﺳﻤﻴﺖ ﻓﻠﺰات ﺳﻨﮕﻴﻦ در ﮔﻴﺎﻫﺎن ﺳﺒﺐ ﺗﻨﺶ اﻛﺴﻴﺪاﺗﻴﻮ ﻣﺮﺑﻮط ﺑﻪ اﻛﺴﻴﺪاﺳﻴﻮن ﭘﺮوﺗﺌﻴﻦ ﻫﺎ و ﭼﺮ ﺑﻲ ﻫﺎی ﻏﺸﺎ و ﻫﻢ ﭼﻨﻴﻦ ﺳﺒﺐ
آﺳﻴﺐ وﺳﻤﻴﺖ DNA ﻣﻲ ﮔﺮدد.در اﻳﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﺑﺮرﺳﻲ آﺳﻴﺐ ژﻧﺘﻴﻜﻲ ﺣﺎﺻﻞ از ﺗﻨﺶ 8 ﻏﻠﻈﺖ ﻣﺘﻔﺎوت ﻧﻴﺘﺮات ﻧﻘﺮه در ﻣﻘﺎﻳﺴﻪ ﺑﺎ ﺷﺎﻫﺪ ﺑﺮ روی 4 ﺟﻤﻌﻴﺖ زﻳﺮه ﺳﺒﺰ ﺷﺎﻣﻞ ﺟﻤﻌﻴﺖ ﻫﺎی ﻛﺮﻣﺎن، ﻳﺰد، ﺧﺮاﺳﺎن ﺟﻨﻮﺑﻲ و اﺻﻔﻬﺎن ﭘﺮداﺧﺘﻪ ﺷﺪ)ﻏﻠﻈﺖ ﻫﺎ ﺑﻪ ﺗﺮﺗﻴﺐ 300،450،600،750،900،1050،1020،1200 ﻣﻴﻜﺮو ﻟﻴﺘﺮ دراﻳﻦ آزﻣﺎﻳﺶ از9 ﻣﺎرﻛﺮISSR ﺑﺮای ﺗﺸﺨﻴﺺ ﺗﻐﻴﻴﺮات ژﻧﺘﻴﻜﻲ اﻳﺠﺎد ﺷﺪه ﻣﺒﻨﻲ ﺑﺮ ﺣﻀﻮر ﻳﺎ ﻋﺪم ﺣﻀﻮر ﺑﺎﻧﺪ اﺳﺘﻔﺎده ﺷﺪ.ﺳﭙﺲ ﻧﺘﺎﻳﺞ ﺑﺎ اﺳﺘﻔﺎده از دوﻓﺮﻣﻮل BSI و GTSﻛﻪ ﺑﻪ ﺗﺮﺗﻴﺐ ﺑﺮ ﻣﺒﻨﺎی ﺑﺎﻧﺪﻫﺎی ﻣﺸﺘﺮک ﻣﺸﺎﻫﺪه ﺷﺪه ﺑﻴﻦ ﻫﺮ ﻏﻠﻈﺖ وﺷﺎﻫﺪ و ﺑﺎﻧﺪﻫﺎی ﭘﻠﻲ ﻣﻮرﻓﻴﻚ ﻫﺮ ﻧﻤﻮﻧﻪ ﻫﺴﺘﻨﺪ ﺑﻴﺎن ﺷﺪ. ﻧﺘﺎﻳﺞ ﺑﺪﺳﺖ آﻣﺪه از ﺷﺎﺧﺺ BSI در اﻳﻦ 4 ﺟﻤﻴﻌﺖ ﻣﻮرد ﺗﻨﺶ ﻧﺸﺎن داد ﻛﻪ ﺑﺎ اﻓﺰاﻳﺶ ﻏﻠﻈﺖ ﻧﻴﺘﺮات ﻧﻘﺮه ﻋﺪد اﻳﻦ ﺷﺎﺧﺺ ﺑﻪ ﺻﻔﺮ ﻧﺰدﻳﻚ ﻣﻲ ﺷﻮد ﻛﻪ ﺑﻴﺎﻧﮕﺮ اﻳﺠﺎد ﺗﻔﺎوت ژﻧﺘﻴﻜﻲ ﻧﻤﻮﻧﻪ ی ﺗﻨﺶ دﻳﺪه ﺑﺎ ﺷﺎﻫﺪ اﺳﺖ. ﻫﻢ ﭼﻨﻴﻦ در ﺑﺮرﺳﻲ اﻳﻦ ﺷﺎﺧﺺ ﺑﺮ ﻣﺒﻨﺎی ﭘﺮاﻳﻤﺮ ﻫﺎ،ﭘﺮاﻳﻤﺮ 1و 9 ﺗﻔﺎوت ﺑﻴﺸﺘﺮی را ﻧﺴﺒﺖ ﺑﻪ ﺳﺎﻳﺮ ﭘﺮاﻳﻤﺮﻫﺎ ﺑﺮ اﺳﺎس اﻳﻦ ﺷﺎﺧﺺ اﻳﺠﺎد ﻛﺮدﻧﺪ. ﺑﺎ اﻗﺰاﻳﺶ ﻏﻠﻈﺖ ﻣﻴﺰان GTS ﻛﻪ ﺑﻴﺎﻧﮕﺮ ﻣﻴﺰان ﺗﻐﻴﺮات اﻟﮕﻮی ISSR ﻳﺎ ﺑﻌﺒﺎرﺗﻲ ﻣﻴﺰان ﭘﻠﻲ ﻣﻮرﻓﻴﺴﻢ ﻫﺴﺖ ﻛﺎﻫﺶ ﭘﻴﺪا ﻣﻲ ﻛﻨﺪ. اﻟﺒﺘﻪ اﻳﻦ ﻛﺎﻫﺶ در ﻏﻠﻈﺘﻲ ﺧﺎص ﻧﺴﺒﺖ ﺑﻪ ﺷﺎﻫﺪ ﺧﻴﻠﻲ زﻳﺎد ﻫﺴﺖ و ﺑﻌﺪ ازن در ﻏﻠﻈﺖ ﻫﺎی دﻳﮕﺮ ﻳﻚ روﻧﺪ ﺛﺎﺑﺖ را ﻧﺸﺎن ﻣﻲ دﻫﺪ.
آﺳﻴﺐ وﺳﻤﻴﺖ DNA ﻣﻲ ﮔﺮدد.در اﻳﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﺑﺮرﺳﻲ آﺳﻴﺐ ژﻧﺘﻴﻜﻲ ﺣﺎﺻﻞ از ﺗﻨﺶ 8 ﻏﻠﻈﺖ ﻣﺘﻔﺎوت ﻧﻴﺘﺮات ﻧﻘﺮه در ﻣﻘﺎﻳﺴﻪ ﺑﺎ ﺷﺎﻫﺪ ﺑﺮ روی 4 ﺟﻤﻌﻴﺖ زﻳﺮه ﺳﺒﺰ ﺷﺎﻣﻞ ﺟﻤﻌﻴﺖ ﻫﺎی ﻛﺮﻣﺎن، ﻳﺰد، ﺧﺮاﺳﺎن ﺟﻨﻮﺑﻲ و اﺻﻔﻬﺎن ﭘﺮداﺧﺘﻪ ﺷﺪ)ﻏﻠﻈﺖ ﻫﺎ ﺑﻪ ﺗﺮﺗﻴﺐ 300،450،600،750،900،1050،1020،1200 ﻣﻴﻜﺮو ﻟﻴﺘﺮ دراﻳﻦ آزﻣﺎﻳﺶ از9 ﻣﺎرﻛﺮISSR ﺑﺮای ﺗﺸﺨﻴﺺ ﺗﻐﻴﻴﺮات ژﻧﺘﻴﻜﻲ اﻳﺠﺎد ﺷﺪه ﻣﺒﻨﻲ ﺑﺮ ﺣﻀﻮر ﻳﺎ ﻋﺪم ﺣﻀﻮر ﺑﺎﻧﺪ اﺳﺘﻔﺎده ﺷﺪ.ﺳﭙﺲ ﻧﺘﺎﻳﺞ ﺑﺎ اﺳﺘﻔﺎده از دوﻓﺮﻣﻮل BSI و GTSﻛﻪ ﺑﻪ ﺗﺮﺗﻴﺐ ﺑﺮ ﻣﺒﻨﺎی ﺑﺎﻧﺪﻫﺎی ﻣﺸﺘﺮک ﻣﺸﺎﻫﺪه ﺷﺪه ﺑﻴﻦ ﻫﺮ ﻏﻠﻈﺖ وﺷﺎﻫﺪ و ﺑﺎﻧﺪﻫﺎی ﭘﻠﻲ ﻣﻮرﻓﻴﻚ ﻫﺮ ﻧﻤﻮﻧﻪ ﻫﺴﺘﻨﺪ ﺑﻴﺎن ﺷﺪ. ﻧﺘﺎﻳﺞ ﺑﺪﺳﺖ آﻣﺪه از ﺷﺎﺧﺺ BSI در اﻳﻦ 4 ﺟﻤﻴﻌﺖ ﻣﻮرد ﺗﻨﺶ ﻧﺸﺎن داد ﻛﻪ ﺑﺎ اﻓﺰاﻳﺶ ﻏﻠﻈﺖ ﻧﻴﺘﺮات ﻧﻘﺮه ﻋﺪد اﻳﻦ ﺷﺎﺧﺺ ﺑﻪ ﺻﻔﺮ ﻧﺰدﻳﻚ ﻣﻲ ﺷﻮد ﻛﻪ ﺑﻴﺎﻧﮕﺮ اﻳﺠﺎد ﺗﻔﺎوت ژﻧﺘﻴﻜﻲ ﻧﻤﻮﻧﻪ ی ﺗﻨﺶ دﻳﺪه ﺑﺎ ﺷﺎﻫﺪ اﺳﺖ. ﻫﻢ ﭼﻨﻴﻦ در ﺑﺮرﺳﻲ اﻳﻦ ﺷﺎﺧﺺ ﺑﺮ ﻣﺒﻨﺎی ﭘﺮاﻳﻤﺮ ﻫﺎ،ﭘﺮاﻳﻤﺮ 1و 9 ﺗﻔﺎوت ﺑﻴﺸﺘﺮی را ﻧﺴﺒﺖ ﺑﻪ ﺳﺎﻳﺮ ﭘﺮاﻳﻤﺮﻫﺎ ﺑﺮ اﺳﺎس اﻳﻦ ﺷﺎﺧﺺ اﻳﺠﺎد ﻛﺮدﻧﺪ. ﺑﺎ اﻗﺰاﻳﺶ ﻏﻠﻈﺖ ﻣﻴﺰان GTS ﻛﻪ ﺑﻴﺎﻧﮕﺮ ﻣﻴﺰان ﺗﻐﻴﺮات اﻟﮕﻮی ISSR ﻳﺎ ﺑﻌﺒﺎرﺗﻲ ﻣﻴﺰان ﭘﻠﻲ ﻣﻮرﻓﻴﺴﻢ ﻫﺴﺖ ﻛﺎﻫﺶ ﭘﻴﺪا ﻣﻲ ﻛﻨﺪ. اﻟﺒﺘﻪ اﻳﻦ ﻛﺎﻫﺶ در ﻏﻠﻈﺘﻲ ﺧﺎص ﻧﺴﺒﺖ ﺑﻪ ﺷﺎﻫﺪ ﺧﻴﻠﻲ زﻳﺎد ﻫﺴﺖ و ﺑﻌﺪ ازن در ﻏﻠﻈﺖ ﻫﺎی دﻳﮕﺮ ﻳﻚ روﻧﺪ ﺛﺎﺑﺖ را ﻧﺸﺎن ﻣﻲ دﻫﺪ.
کلیدواژه ها
Title
study the stability of genomic DNA in Cuminum cyminum in response to silver nitrate stress by using ISSR markers
Authors
Abstract
The toxicity of heavy metals in plants causes oxidative stress related to fat oxidation and membrane proteins and also causes DNA damage and toxicity. In our study, 4populations of Cuminum cyminum from Kerman, South Khorasan, Yazd and Isfahan were placed under stress of 8 different concentrations of silver nitrate. Then for evaluating the changes, 9 ISSR markers were used. Finally, the genetic differences were evaluated according to the control within any population by using two formula BSI and GTS that respectively, are based on common bands were observed between control and each concentration and Polymorphic bands of each sample.Results from BSI index in the four populations showed that by increasing the concentration of silver nitrate value this index is close to zero , which indicates that genetic differences between sample and control.Also check the index-based primers, primer 1 and 9 showed more genetic differences than others.With increasing concentrations, GTS index, which represents the amount or the rate of change of ISSR patterns of polymorphism is reduced.
Keywords
BSI, GTS, Cuminum cyminum, silver nitrate, ISSR