اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮﻫﺎی ISSR در ﺑﺮرﺳﻲ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ اﺳﺘﺒﺮق

Identification and classification of milkweed plants in different heights using ISSR markers were studied. For this purpose, next to DNA extraction, optimization of Conditions and Different concentrations of materials used in the PCR, 9 primers were used individually and in combination. in total, 259 Pieces were amplified that, 106 of them were polymorphic fragments. Statistical analysis was performed using jakard method and NTSYS Ver.2.02 software. PIC levels for different markers , were variable from 0.37 for ISSR2 marker to 0/46 for ISSR5 and ISSR9 markers. 14 samples were classified into three groups that 2 groups have 6 samples and 1 group has 3 samles. In total, ISSR technique is useful for classifying milkweed plants and perhaps more helpful for identification and grouping of samples, using more markers. Considerable genetic variation exists within kerman native milkweeds and Milkweed examples are different in levels of genetic diversity. Likely The main reason for the creation of genetic distance Between samples are Environmental factors and sexual reproduction.