بررسی ترکیب پذیری ژنتیکی چند رقم و لاین نرعقیم توتون گرمخانه‌ای و معرفی بهترین هیبریدها

To get the best hybrids with maximum heterosis, a genetic design should be performed to evaluate the genetic combining ability (GCA) of parental genotypes. This research was performed on six newly breeded varieties (Totem 321, Totem 322, Totem 323, Totem 324, Totem 325 and Totem 341) and six Iranian cytoplasm male sterile (CMS) lines with line X tester techniques. Treatments, including 36 hybrids and 12 parents, were planted as a simple lattice design with two replications in Tirtash Research and Education Center in 2010. Green and cured leaf yield, average price, gross income, number of leaves, leaf length and width, sugar, nicotine and potassium of cutters were measured. The results of variance analysis in the simple lattice design showed significant difference for many trates. Analysis of the effect of crosses to their components, including lines, testers and line × tester interaction also showed that the lines were not significantly different. The interaction between the lines and testers for green and cured leaf yield were significant, this could indicate a different reaction of lines in combination with different testers. GCA analysis for lines and testers showed that Totem 323 and Totem 325 varieties, also Coker 347 CMS1, Coker 347 CMS2, and K 326 CMS2 testers have positive and significant GCA. Therefore, these varieties could be used as parent in breeding programs. Also, six hybrids whose parents have positive and significant GCA and their crosses had positive and significant specific combining ability (SCA) and also had positive and high heterosis compared to best parent, can be used for producing male sterile hybrids.