ﺑﺮرﺳﻲ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ و رواﺑﻂ ﻓﻴﻠﻮژﻧﺘﻴﻜﻲ ﺑﺮﺧﻲ ﮔﻮﻧﻪﻫﺎی زﻋﻔﺮان ﺑﺎ اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮﻫﺎی RAPD

Saffron (Crocus sativa L.) is a native plant of Iran with various medical and food properties. It also has an important role in terms of economical issues in Iran. One of the major applications of molecular techniques to investigate and estimate the level of genetic diversity in germplasm and populations, it is ideal for use in breeding and management of plant genetic resources is maintained. The diversity and genetic relationship of 25 samples of cultivated and wild species of crocus C. sativus, C. speciosus, C. cansellatuse, C. haussknechii and C. michelsonii were studied using several RAPD markers. Eleven RAPD primers produced 90 bands it scored. Average polymorphism of RAPD markers obtained 77. 95 %. Results of analysis of molecular variance showed that genetic variation between populations and within populations were significant. AMOVA analysis showed that the variation between the species, between different populations, and within populations accounted for 36, and 64% of the total variation, respectively. Mean polymorphic information content (PIC) of the markers was 0.257 and ranged between 0.119 and 0.313. The results showed that there is a lot of variation between and within species crocus that can be used to value the plant breeding programs.