ﺗﻌﻴﻴﻦ اﻧﺪازه ﻣﻨﺎﺳﺐ ﻏﻨﭽﻪ و ﻧﻮع ﻛﺮﺑﻮﻫﻴﺪرات در ﻣﺤﻴﻂ ﻛﺸﺖ ﺟﻬﺖ ﻛﺎﻟﻮسزاﻳﻲ و روﻳﺎنزاﻳﻲ ﮔﺎﻣﺘﻲ ﮔﻴﺎه ﮔﺎوزﺑﺎن اروﭘﺎﻳﻲ (Borago officinalis L.) از ﻃﺮﻳﻖ ﻛﺸﺖ ﺑﺴﺎک

This study is directed on specifying of suitable size of bud and type of carbohydrate in medium on callogenesis and gametic embryogenesis in cultured anther of borage (Borago officinalis L.). A factorial experiment based on completely randomized design was used in this study. Three size of bud (5, 7 and 9 mm) were used as first factor and two type of carbohydrate that there are in the medium, including a concentration of maltose and sucrose (30 g lit-1) were considered as second factor. Result showed significant differences among various sizes of bud and type of carbohydrate for callogenesis and gametic embryogenesis from anther culture of borage. Used of bud with 5 and 7mm length, showed the highest callogenesis percentage and mean number of embryo per anther in cultured anther of borage and also the used of maltose in medium, showed the highest callogenesis percentage in culturing borage,s anther.