بررﺳﻲ اﺛﺮ ﻧﻮع و ﻏﻠﻈﺖ ﻛﺮﺑﻮﻫﻴﺪرات ﻣﻮﺟﻮد در ﻣﺤﻴﻂ ﻛﺸﺖ ﺟﻬﺖ ﻛﺎﻟﻮسزاﻳﻲ ﮔﻴﺎه ﮔﺎوزﺑﺎن اروﭘﺎﻳﻲ (Borago officinalis L) از ﻃﺮﻳﻖ ﻛﺸﺖ ﺑﺴﺎک

AbstractIn this study, the effect of the various type and concentration of carbohydrate on callogenesis from anther culture of borage (Borago officinalis L.) was investigated. A factorial experiment based on completely randomized design was used in this study. Two type of carbohydrate ( maltose and sucrose) were used as first factor and the three concentration of carbohydrate (30, 60 and 90 g lit-1 ) were considered as second factor. The results of used of various carbohydrate treetments showed that there are significant differences between the carbohydrate of medium for type and concentration , for callogenesis induction in culturing borage,s anther. The used of maltose in medium, has a positive effect of callogenesis induction rate in culturing borage,s anther. Also the used of 30 g lit-1 maltose in the medium , showed the highest callogenesis percentage in anther culture of borage.