اثر تیمارهای هورمونی مختلف و نوع ریز نمونه بر ریز ازدیادی گیاه دارویی به لیمو

Lippia Citrodoria Linn. is an important medicinal herb belonging to the family Verbenaceae. This study investigated a method for rapid micropropagation Lippia Citrodoria through node propagation from terminal and auxiliary nodal explants . Th explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyl amino purine (BAP; 0, 0.5, 1 and 2 mg/l) alone or in combination with indole 3- butyric acid (IBA) or (alpha)-naphthalene acetic acid (NAA) in concentration of 0,0.1 and 0.5 mg/l for micropropagtion. The length and number of shoots and roots were examined after 1 month of culture. Through our work, we have found that auxiliary node explants cultured on MS Basal media in combination with 1 mg/l BAP + 0.05 mg/l of NAA + 2 gr/l activated charcoal gives the best result for the length and number of shoots auxiliary shoots. Maximum number and length of roots were observed on MS media without any plant grow regulators and in combination with 2 gr/l l activated charcoal. The plantlet were acclimated and then transferred to green house. This in vitro propagation protocol should be useful for conservation as well as mass propagation of this medicinal plant.