ﺑﺮرﺳﻲ ﻣﻴﺰان ﭘﺮاﻛﺴﻴﺪاﺳﻴﻮن ﭼﺮﺑﻴﻬﺎ و ﻓﻌﺎﻟﻴﺖ آﻧﺰﻳﻢ ﺳﻮﭘﺮ اﻛﺴﻴﺪ دﺳﻤﻮﺗﺎز دو رﻗﻢ ﺣﺴﺎس و ﻣﺘﺤﻤﻞ ﺑﻪ ﺷﻮری ﮔﻨﺪم در ﻣﺮاﺣﻞ ﻣﺨﺘﻠﻒ رﺷﺪ در اﺛﺮ آﺑﻴﺎری ﺑﺎ آب ﺷﻮر
پذیرفته شده برای پوستر
عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1اﺳﺘﺎدﻳﺎر ﭘﮋوﻫﺸﻜﺪه ﻛﺸﺎورزی ﻫﺴﺘﻪ ای، ﭘﮋوﻫﺸﮕﺎه ﻋﻠﻮم و ﻓﻨﻮن ﻫﺴﺘﻪ ای
2کارﺷﻨﺎس ﭘﮋوﻫﺸﻜﺪه ﻛﺸﺎورزی ﻫﺴﺘﻪ ای، ﭘﮋوﻫﺸﮕﺎه ﻋﻠﻮم و ﻓﻨﻮن ﻫﺴﺘﻪ ای
چکیده
ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﻲ اﺛﺮ ﺷﻮری در ﺳﻪ ﻣﺮﺣﻠﻪ رﺷﺪی ﺑﺮ ﻓﻌﺎﻟﻴﺖ آﻧﺰﻳﻢ ﺳﻮﭘﺮ اﻛﺴﻴﺪ دﺳﻤﻮﺗﺎز وﻣﻴﺰان ﻣﺎﻟﻮﻧﺪآﻟﺪﺋﻴﺪ دو رﻗﻢ ﮔﻨﺪم، آزﻣﺎﻳﺸﻲ ﺑﻪ ﺻﻮرت اﺳﭙﻠﻴﺖ ﻓﺎﻛﺘﻮرﻳﻞ در ﻗﺎﻟﺐ ﻃﺮح ﻛﺎﻣﻼً ﺗﺼﺎدﻓﻲ و ﺑﺎ ﺳﻪ ﺗﻜﺮار در ﮔﻠﺨﺎﻧﻪ ﺗﺤﻘﻴﻘﺎﺗﻲ ﭘﮋوﻫﺸﻜﺪه ﻛﺸﺎورزی ﻫﺴﺘﻪ ای ﻛﺮج اﺟـﺮاء ﺷﺪ. ﺗﻴﻤﺎر ﻣﺮﺣﻠﻪ رﺷﺪ )ﭘﻨﺠﻪ زﻧﻲ، ﮔﺮده اﻓﺸﺎﻧﻲ و 10 روز ﭘﺲ از ﮔﺮده اﻓﺸﺎﻧﻲ( در ﭘﻼت اﺻﻠﻲ و ﺗﺮﻛﻴﺐ دو رﻗﻢ ﮔﻨﺪم ﺑﻪ ﻧﺎﻣﻬـﺎی ﺑـﻢ )ﻣﻘﺎوم ﺑﻪ ﺗﻨﺶ ﺷﻮری( و ﺗﺠﻦ )ﺣﺴﺎس ﺑﻪ ﺗﻨﺶ ﺷﻮری( ﺑﻪ ﻫﻤﺮاه ﭘﻨﺞ ﺗﻴﻤـﺎر ﺷـﻮری 0، 6، 8، 10 و 12 دﺳـﻲ زﻳﻤـﻨﺲ ﺑـﺮ ﻣﺘـﺮ( ﺑـﻪ ﺻﻮرت ﻓﺎﻛﺘﻮرﻳﻞ در ﭘﻼتﻫﺎی ﻓﺮﻋﻲ در ﻧﻈﺮ ﮔﺮﻓﺘﻪ ﺷﺪﻧﺪ. ﻧﺘﺎﻳﺞ آزﻣﺎﻳﺶ ﻧﺸﺎن داد اﻋﻤﺎل ﺗـﻨﺶ ﺷـﻮری در ﻃـﻮل ﻓﺼـﻞ رﺷـﺪ ﺳـﺒﺐ ﻛﺎﻫﺶ ﻣﻘﺪار ﻣﺎﻟﻮﻧﺪآﻟﺪﺋﻴﺪ )MDA( ﮔﺮدﻳﺪ. ﻓﻌﺎﻟﻴﺖ آﻧﺰﻳﻢ ﺳﻮﭘﺮ اﻛﺴﻴﺪ دﺳﻤﻮﺗﺎز (SOD) ﻧﻴﺰ در ﺷﺮاﻳﻂ آﺑﻴﺎری ﺑﺎ آب ﺷﻮر ﺑﻪ ﻃﻮر ﻣﻌﻨـﻲ داری اﻓﺰاﻳﺶ ﻳﺎﻓﺖ. ﻧﺘﺎﻳﺞ ﺣﺎﻛﻲ از آن ﺑﻮد ﻛﻪ رﻗﻢ ﻣﻘﺎوم ﺑﻢ ﻛﻪ در ﺷﺮاﻳﻂ ﺗـﻨﺶ ﺷـﻮری از ﻣﺎﻟﻮﻧﺪآﻟﺪﺋﻴـﺪ ﭘـﺎﺋﻴﻦ ﺗـﺮی ﺑﺮﺧـﻮردار ﺑـﻮد،ﺑﻴﺸﺘﺮﻳﻦ ﻓﻌﺎﻟﻴﺖ آﻧﺰﻳﻢ ﺳﻮﭘﺮ اﻛﺴﻴﺪ دﺳﻤﻮﺗﺎز را ﻧﻴﺰ در ﻣﻘﺎﻳﺴﻪ ﺑﺎ رﻗﻢ ﺗﺠﻦ از ﺧﻮد ﻧﺸﺎن داد. ﺑﻨﺎﺑﺮاﻳﻦ ﺑﻪ ﻧﻈﺮ ﻣﻲ رﺳﺪ ارزﻳـﺎﺑﻲ واﻛـﻨﺶﺑﺮﺧﻲ از ﺻﻔﺎت ﻓﻴﺰﻳﻮﻟﻮژﻳﻚ در ﻣﻬﻢ ﺗﺮﻳﻦ ﻣﺮاﺣﻞ رﺷﺪی ﮔﻴﺎه در ﺗﻌﻴﻴﻦ ﻣﻴﺰان ﺗﺤﻤﻞ ﺑﻪ ﺷﻮری ارﻗﺎم ﮔﻨﺪم ﻣﻮﺛﺮ ﺑﺎﺷﺪ
کلیدواژه ها
Title
The Investigation of Lipid peroxidation and Enzyme Superoxide Dismutas Activity in Two Wheat (Triticum aestivum L.) Cultivars at Different Growth Stages Under Irrigation Water Salinity
Authors
Abstract
In order to study the effect of salt stress at different growth stage on physiological characteristics and enzyme superoxide dismutas (SOD) activity in wheat genotypes a greenhouse experiment was conducted at the Agricultural Research Green house, Agricultural, Medical and Industrial Research School, Nuclear Science and Technology Research Institute of Karaj. A split factorial design on the basis of randomized complete desing with three replications was applied. Growth stage (tillering, anthesis and 10 days after anthesis) served as main plots and a combination of five salinity treatments (0, 6, 8, 10 and 12 ds/m) and two wheat cultivars, Bam (resistance) and Tajan (susceptible to salt stress) served as subplots. Salt stress at different growth stages resulted in a decrease in relative water content. Although salinity increased MDA, content in all cultivars. The antioxidant enzyme superoxide dismutas (SOD) activity also increased under salt stress. Bam cultivar, which had higher SOD activity, had the lower MDA content than Tajan cultivar under salt stress. So the evaluation of some physiological and biochemical characteristics at the most important growth stages of wheat may be useful for screening the salinity tolerance genotypes.
Keywords
salinity, SOD Activity MDA contents