اﺛﺮ ﺗﻨﺶ ﺷﻮری ﺑﺮ ﺧﺼﻮﺻﻴﺎت ﺑﻴﻮﺷﻴﻤﻴﺎﻳﻲ ﮔﻴﺎه داروﻳﻲ ﺷﻨﺒﻠﻴﻠﻪ (Trigonella foenum – graecum L)

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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ ﻋﻠﻮم ﺑﺎﻏﺒﺎﻧﻲ، داﻧﺸﻜﺪه ﻛﺸﺎورزی، داﻧﺸﮕﺎه ﻓﺮدوﺳﻲ ﻣﺸﻬﺪ
2اﺳﺘﺎد ﮔﺮوه ﻋﻠﻮم ﺑﺎﻏﺒﺎﻧﻲ، داﻧﺸﻜﺪه ﻛﺸﺎورزی،داﻧﺸﮕﺎه ﻓﺮدوﺳﻲ ﻣﺸﻬﺪ
3اﺳﺘﺎدﻳﺎر ﮔﺮوه ﻋﻠﻮم ﺑﺎﻏﺒﺎﻧﻲ، داﻧﺸﻜﺪه ﻛﺸﺎورزی، داﻧﺸﮕﺎه ﻓﺮدوﺳﻲ ﻣﺸﻬﺪ
چکیده
ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﻲ ﺗﺎﺛﻴﺮ ﺳﻄﻮح ﻣﺨﺘﻠﻒ ﺷﻮری ﺑﺮ وﻳﮋﮔﻴﻬﺎی ﺑﻴﻮﺷﻴﻤﻴﺎﻳﻲ ﮔﻴﺎه داروﻳﻲ ﺷﻨﺒﻠﻴﻠﻪ )Trigonella foenom- graecum
L.( آزﻣﺎﻳﺸﻲ ﺑﻪ ﺻﻮرت ﻓﺎﻛﺘﻮرﻳﻞ در ﻗﺎﻟﺐ ﻃﺮح ﻛﺎﻣﻼً ﺗﺼﺎدﻓﻲ ﺑﺎ ﺳﻪ ﺗﻜﺮار در ﺧﺎرج از ﮔﻠﺨﺎﻧﻪ در ﺳﺎل 1392 اﻧﺠﺎم ﺷﺪ. ﺗﻴﻤﺎرﻫﺎی آزﻣﺎﻳﺸﻲ ﺷﺎﻣﻞ ﻫﺸﺖ ﺗﻮده ﺑﻮﻣﻲ ﺷﻨﺒﻠﻴﻠﻪ )اﺻﻔﻬﺎن، ﺗﺒﺮﻳﺰ، ﻫﻤﺪان، ﺳﺎری، ﭼﺎﻟﻮس، آﻣﻞ، ﻣﺸﻬﺪ و ﻳﺎﺳﻮج( و 4 ﺳﻄﺢ ﺷﻮری )180،120، 160، 0 ﻣﻴﻠﻲ ﻣﻮﻻر ﻛﻠﺮﻳﺪ ﺳﺪﻳﻢ( ﺑﻮد. اﺛﺮ ﺳﻄﻮح ﻣﺨﺘﻠﻒ ﺷﻮری ﺑﺮ ﻣﺤﺘﻮای رﻃﻮﺑﺖ ﻧﺴﺒﻲ، ﭘﺎﻳﺪاری ﻏﺸﺎ، ﻛﻠﺮوﻓﻴﻞ a و ﭘﺮوﻟﻴﻦ ﺑﺮگ ﻣﻌﻨﻲ دار )0/01≤p( ﺑﻮد. ﺳﻄﻮح ﻣﺨﺘﻠﻒ ﺷﻮری ﺑﺎﻋﺚ ﻛﺎﻫﺶ ﻣﻌﻨﻲ دار ﺗﻤﺎم ﺻﻔﺎت اﻧﺪازه ﮔﻴﺮی ﺷﺪه ﺷﺪ. ﺑﻪ ﻃﻮری ﻛﻪ، ﻣﻘﺎﻳﺴﻪ ﻣﻴﺎﻧﮕﻴﻦ ﺗﻴﻤﺎرﻫﺎ ﻧﺸﺎن داد ﻛﻪ ﺑﻴﺸﺘﺮﻳﻦ ﻣﻴﺰان اﻳﻦ ﺻﻔﺎت ﻣﺮﺑﻮط ﺑﻪ ﺗﻴﻤﺎر ﺷﺎﻫﺪ ﺻﻔﺮ ﻣﻴﻠﻲ ﻣﻮﻻر( ﺑﻮد. ﺗﻮده ﻫﺎی اﺻﻔﻬﺎن، ﻣﺸﻬﺪ و ﭼﺎﻟﻮس ﻧﺴﺒﺖ ﺑﻪ ﺳﺎﻳﺮ ﺗﻮده ﻫﺎ در ﺑﻴﺸﺘﺮ ﺻﻔﺎت اﻧﺪازه ﮔﻴﺮی ﺷﺪه ﺑﺎﻻﺗﺮﻳﻦ ﻣﻘﺪار را ﻧﺸﺎن دادﻧﺪ. ﺑﺮ اﺳﺎس ﻧﺘﺎﻳﺞ ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، ﺑﻪ ﻧﻈﺮ ﻣﻲ رﺳﺪ ﺑﺘﻮان از اﻳﻦ ﺳﻪ ﺗﻮده ﺑﻪ ﻋﻨﻮان ﺗﻮده ﻫﺎی ﺣﺎوی ژن ﻫﺎی ﻣﻘﺎوﻣﺖ ﺑﻪ ﺷﻮری ﺑﺮای اﺻﻼح ﺗﻮده ﻫﺎﻳﻲ ﺑﺎ ﭘﺘﺎﻧﺴﻴﻞ ﻋﻤﻠﻜﺮد زﻳﺎداستفاده ﻛﺮد.
کلیدواژه ها
 
Title
Effect of salinity stress on biochemical characteristics of medical plant fenugreek (Trigonella foenum-graecum L.)
Authors
Abstract
In order to investigate the effect of different salinity levels on biochemical characteristics of medical plant fenugreek (Trigonella foenum-graecum L.) a factorial experiment was conducted in a completely randomized design with three replicates in the outside of greenhouse in 2013. Experimental treatments were Combination of eight fenugreek landrace (Isfahan, Tabriz, Hamedan, Sari ,Challous, Amol ,Mashhad and Yasooj) and four levels of salinity stress (0, 60, 120 and 180 Mm NaCl). Effect of different salinity levels on relative humidity, membrane stability, chlorophyll a and prolline of leaf significant differences (P≤0/01). Different levels of salinity led to significant reductions in all of measured traits. Comparison of treatment means revealed that the maximum amount of measured characteristics belonged to control (0 Mm). The Isfahan, Mashhad and Challous landraces showed the highest amount in most of the characteristics in comparison with others. According to current results, three landraces can be considered as the landraces which contains genes of salt tolerance used to modify landraces with high potential yield.
Keywords
fenugreek, salinity, Chlorophyll, membrane stability (%), prolline