ﺮرﺳﻲ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ ژﻧﻮﺗﻴﭗﻫﺎی ﻣﺨﺘﻠﻒ ﺑﺮﻧﺞ ﺑﺎ اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮﻫﺎی ﻣﻮﻟﻜﻮﻟﻲ ﺑﻴﻦ رﻳﺰ ﻣﺎﻫﻮارهای (ISSR)
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اصل مقاله (119.81 K)
عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1دانشیار دانشکده کشاورزی دانشگاه گنبدکاووس
2دانشجوی کارشناسی ارشد بیوتکنولوژی کشاورزی دانشگاه پیام نور کرج
چکیده
اﻳﻦ ﺗﺤﻘﻴﻖ ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﻲ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ 59 ژﻧﻮﺗﻴﭗ ﺑﺮﻧﺞ ﺑﺎ اﺳﺘﻔﺎده 10 ﻧﺸﺎﻧﮕﺮ ﻣﻮﻟﻜﻮﻟﻲ ﺑﻴﻦ رﻳﺰ ﻣﺎﻫﻮارهای ISSRاﻧﺠﺎم ﮔﺮﻓﺖ در ﻣﺠﻤﻮع 144 ﺑﺎﻧﺪ ﺑﺎ ﻣﻴﺎﻧﮕﻴﻦ 14/4ﺑﺎﻧﺪ ﺑﻪ ازای ﻫﺮ ﺟﺎﻳﮕﺎه ﻧﺸﺎﻧﮕﺮی اﻳﺠﺎد ﻧﻤﻮد و از 144 ﺑﺎﻧﺪ ﺗﺸﻜﻴﻞ ﺷﺪه 69/17 درﺻﺪ از ﺑﺎﻧﺪﻫﺎ ﭼﻨﺪﺷﻜﻞ ﺑﻮدﻧﺪ. آﻏﺎزﮔﺮ 3-ISSR ﺑﺎ 19 ﺑﺎﻧﺪ وﺗﻮاﻟﻲ آﻏﺎزﮔﺮی (CCTA)4 ﺑﻴﺸﺘﺮﻳﻦ ﺗﻌﺪاد ﺑﺎﻧﺪ و آﻏﺎزﮔﺮ 5-ISSR ﺑﺎ 8 ﺑﺎﻧﺪ و ﺗﻮاﻟﻲ آﻏﺎزﮔﺮی(CCA)4 ﻛﻤﺘﺮﻳﻦ ﺗﻌﺪاد ﺑﺎﻧﺪ را ﺑﻪ ﺧﻮد اﺧﺘﺼﺎص دادﻧﺪ. آﻏﺎزﮔﺮ 10-ISSR ﺑﺎ ﺗﻮاﻟﻲ آﻏﺎزﮔﺮی (GT)6CC ﺑﻴﺸﺘﺮﻳﻦ درﺻﺪ ﭼﻨﺪﺷﻜﻠﻲ)80 درﺻﺪ( و آﻏﺎزﮔﺮ ISSR-9 ﺑﺎ ﺗﻮاﻟﻲ آﻏﺎزﮔﺮی (ACTG)4 ﻛﻤﺘﺮﻳﻦ درﺻﺪ ﭼﻨﺪﺷﻜﻠﻲ داﺷﺘﻨﺪ. ﺑﻴﺸﺘﺮﻳﻦ ﻣﺤﺘﻮای اﻃﻼﻋﺎت ﭼﻨﺪ ﺷﻜﻞ)PIC( را آﻏﺎزﮔﺮ ISSR-7 ﺑﺎ 0/49 و آﻏﺎزﮔﺮﻫﺎﻳﻲ ISSR-9 ،ISSR-8 ،ISSR-2 و (MI 34 )بیشترین شاخص نشانگری / با 8 ISSR- را به خود اختصاص دادند. نشانگر 10 PIC 0 کمترین مقدار / 1
ﻧﺸﺎﻧﮕﺮ4-ISSR ﺑﺎ 28/23 ﻛﻤﺘﺮﻳﻦ ﻣﻘﺪار ﺷﺎﺧﺺ ﻧﺸﺎﻧﮕﺮی را داﺷﺘﻨﺪ. آﻏﺎزﮔﺮ10 -(GT)6 CC) ISSR( ﺑﺎ دارا ﺑﻮدن ﺑﻴﺸﺘﺮﻳﻦ درﺻﺪ ﭼﻨﺪﺷﻜﻠﻲ) 80 درﺻﺪ( و ﺷﺎﺧﺺ ﻧﺸﺎﻧﮕﺮی)38/4 ﻧﺸﺎﻧﮕﺮ ﻣﻨﺎﺳﺐ ﺑﺮای ﺑﺮرﺳﻲ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ ژﻧﻮﺗﻴﭗﻫﺎی ﺑﺮﻧﺞ ﺑﻮد. دﻧﺪروﮔﺮام ﺣﺎﺻﻞ از ﺗﺠﺰﻳﻪ ﺧﻮﺷﻪای ﺑﺎ اﺳﺘﻔﺎده از UPGMA ، ﺑﺮاﺳﺎس ﻧﺸﺎﻧﮕﺮﻫﺎی ISSR ژﻧﻮﺗﻴﭗﻫﺎی ﻣﻮرد ارزﻳﺎﺑﻲ در دو ﮔﺮوه اﺻﻠﻲ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻧﺘﻴﺠﻪ ﮔﺮﻓﺖ ﺷﺪ ژﻧﻮﺗﻴﭗﻫﺎی ﻛﻪ در ﻳﻚ ﮔﺮوه ﻗﺮار ﮔﺮﻓﺘﻨﺪ، ﺑﺎ وﺟﻮد ﺗﻔﺎوتﻫﺎی ﻇﺎﻫﺮی دارای ﺗﻌﺪاد ﺗﻮاﻟﻲﻫﺎی ﺗﻜﺮاری ﻣﺸﺎﺑﻪ ﻫﺴﺘﻨﺪ.
ﻧﺸﺎﻧﮕﺮ4-ISSR ﺑﺎ 28/23 ﻛﻤﺘﺮﻳﻦ ﻣﻘﺪار ﺷﺎﺧﺺ ﻧﺸﺎﻧﮕﺮی را داﺷﺘﻨﺪ. آﻏﺎزﮔﺮ10 -(GT)6 CC) ISSR( ﺑﺎ دارا ﺑﻮدن ﺑﻴﺸﺘﺮﻳﻦ درﺻﺪ ﭼﻨﺪﺷﻜﻠﻲ) 80 درﺻﺪ( و ﺷﺎﺧﺺ ﻧﺸﺎﻧﮕﺮی)38/4 ﻧﺸﺎﻧﮕﺮ ﻣﻨﺎﺳﺐ ﺑﺮای ﺑﺮرﺳﻲ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ ژﻧﻮﺗﻴﭗﻫﺎی ﺑﺮﻧﺞ ﺑﻮد. دﻧﺪروﮔﺮام ﺣﺎﺻﻞ از ﺗﺠﺰﻳﻪ ﺧﻮﺷﻪای ﺑﺎ اﺳﺘﻔﺎده از UPGMA ، ﺑﺮاﺳﺎس ﻧﺸﺎﻧﮕﺮﻫﺎی ISSR ژﻧﻮﺗﻴﭗﻫﺎی ﻣﻮرد ارزﻳﺎﺑﻲ در دو ﮔﺮوه اﺻﻠﻲ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻧﺘﻴﺠﻪ ﮔﺮﻓﺖ ﺷﺪ ژﻧﻮﺗﻴﭗﻫﺎی ﻛﻪ در ﻳﻚ ﮔﺮوه ﻗﺮار ﮔﺮﻓﺘﻨﺪ، ﺑﺎ وﺟﻮد ﺗﻔﺎوتﻫﺎی ﻇﺎﻫﺮی دارای ﺗﻌﺪاد ﺗﻮاﻟﻲﻫﺎی ﺗﻜﺮاری ﻣﺸﺎﺑﻪ ﻫﺴﺘﻨﺪ.
کلیدواژه ها
Title
Assessment of genetic diversity among different genotypes rice using markers ISSR
Authors
Abstract
This study was designed to investigate the genetic diversity of 59 rice genotypes using SSR molecular
markers 10 an ISSR done. A total of 144 bands with an average of 4/14 bands and 144 bands per locus
marker set consists of 69.17 percent of the bands were polymorphic. Primer ISSR-3 with 19 band sequencing
primer (CCTA) 4Maximum number of bands and the primer ISSR-5 with 8 bands and sequencing primer
(CCA)4 minimum number of allocated band. Primer ISSR-10 primer sequences (GT) 6CC highest
percentage of polymorphism (80%) and primer ISSR-9 boot sequence (ACTG) 4 lowest percentage of
polymorphism (55/55) patients. The most polymorphic information content (PIC) of the primer ISSR-7 with
0.49 and primers ISSR-2, ISSR-8, ISSR-9 and ISSR-10 with0.47, the lowest value was accounted for by the
PIC. Markers ISSR-10 with 38.4 highest marker index (MI) and marker ISSR-4 with28.23 had the lowest
indication. Primer ISSR-10 ((GT)6 CC) genotypes having the highest percentage (80 percent) and marker
index (38.4) markers for assessment of genetic diversity of rice genotypes was. dendrogram of cluster
analysis using UPGMA, based on ISSR marker genotype of the two groups were evaluated. Conclude that
genotypes were in a group, despite apparent differences in the number of repetitive sequences are similar
markers 10 an ISSR done. A total of 144 bands with an average of 4/14 bands and 144 bands per locus
marker set consists of 69.17 percent of the bands were polymorphic. Primer ISSR-3 with 19 band sequencing
primer (CCTA) 4Maximum number of bands and the primer ISSR-5 with 8 bands and sequencing primer
(CCA)4 minimum number of allocated band. Primer ISSR-10 primer sequences (GT) 6CC highest
percentage of polymorphism (80%) and primer ISSR-9 boot sequence (ACTG) 4 lowest percentage of
polymorphism (55/55) patients. The most polymorphic information content (PIC) of the primer ISSR-7 with
0.49 and primers ISSR-2, ISSR-8, ISSR-9 and ISSR-10 with0.47, the lowest value was accounted for by the
PIC. Markers ISSR-10 with 38.4 highest marker index (MI) and marker ISSR-4 with28.23 had the lowest
indication. Primer ISSR-10 ((GT)6 CC) genotypes having the highest percentage (80 percent) and marker
index (38.4) markers for assessment of genetic diversity of rice genotypes was. dendrogram of cluster
analysis using UPGMA, based on ISSR marker genotype of the two groups were evaluated. Conclude that
genotypes were in a group, despite apparent differences in the number of repetitive sequences are similar
Keywords
rice, Genotypes critical, Indicator index (MI), Markers ISSR