ﺑﺮرﺳﻲ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ ﻻﻳﻦﻫﺎی ﭘﻴﺸﺮﻓﺘﻪ ﮔﻨﺪم دوروم ﺑﺎ اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮ RAPD
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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1دانشجو رشته اصلاح نباتات دانشگاه محقق اردبیلی،
2دانشیار دانشگاه محقق اردبیلی
3عضو هیات علمی موسسه تحقیقات کشاورزی دیم کشور- ایستگاه گچساران
چکیده
اوﻟﻴﻦ ﮔﺎم در ﺑﺮﻧﺎﻣﻪﻫﺎی ﺑﻪ ﻧﮋادی، داﺷﺘﻦ اﻃﻼﻋﺎت ﻛﺎﻓﻲ در ﻣﻮرد ﺗﻨﻮع ژﻧﺘﻴﻜﻲ ﺑﻴﻦ ژﻧﻮﺗﻴﭗﻫﺎی ﻣﻮﺟﻮد اﺳﺖ. ﺑﻪ ﻫﻤﻴﻦ ﻣﻨﻈﻮر در اﻳﻦ ﻣﻄﺎﻟﻌﻪ ﺗﻨﻮع ژﻧﺘﻴﻜﻲ 20 ﻻﻳﻦ ﮔﻨﺪم دوروم ﺑﺎ اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮ RAPD ﻣﻮرد ﺑﺮرﺳﻲ ﻗﺮار ﮔﺮﻓﺖ. 14 آﻏﺎزﮔﺮ از 50 آﻏﺎزﮔﺮ ﻣﻮرد اﺳﺘﻔﺎده، 108 ﻧﻮار ﭼﻨﺪ ﺷﻜﻞ ﺗﻮﻟﻴﺪ ﻛﺮدﻧﺪ. درﺻﺪ ﭼﻨﺪ ﺷﻜﻠﻲ ﺑﺮای ﻛﻠﻴﻪ آﻏﺎزﮔﺮﻫﺎ 100 ﺑﻮد. ﻛﻤﺘﺮﻳﻦ ﺗﻌﺪاد ﻧﻮار ﻣﺮﺑﻮط ﺑﻪ آﻏﺎزﮔﺮ 25 و ﺑﻴﺸﺘﺮﻳﻦ ﺗﻌﺪاد ﻣﺮﺑﻮط ﺑﻪ آﻏﺎزﮔﺮﻫﺎی 30 و 33 ﺑﻮد. ﺑﺎﻻ ﺑﻮدن ﻣﻌﻴﺎرﻫﺎی ﺗﻌﺪاد اﻟﻞ ﻣﻮﺛﺮ، ﺗﻨﻮع ژﻧﻲ ﻧﻲ، ﺷﺎﺧﺺ ﺷﺎﻧﻮن و ﻣﻴﺰان PIC
ﺑﺮای آﻏﺎزﮔﺮ 19 ﻧﺸﺎن دﻫﻨﺪه ﻛﺎراﻳﻲ ﺑﺎﻻی اﻳﻦ آﻏﺎزﮔﺮ در ﺗﻤﺎﻳﺰ ﻻﻳﻦﻫﺎی ﮔﻨﺪم دوروم ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻣﻲﺑﺎﺷﺪ. ﺗﺠﺰﻳﻪ ﺧﻮﺷﻪای ﺑﻪ روش UPGMA و ﺑﺎ اﺳﺘﻔﺎده از ﺿﺮﻳﺐ ﺗﺸﺎﺑﻪ داﻳﺲ اﻧﺠﺎم ﺷﺪ. ﻻﻳﻦﻫﺎی ﻣﻮرد ﺑﺮرﺳﻲ در 3ﺧﻮﺷﻪ ﺟﺪاﮔﺎﻧﻪ ﻃﺒﻘﻪﺑﻨﺪی ﮔﺮدﻳﺪ. ﻫﻢﭼﻨﻴﻦ،ﻧﺘﺎﻳﺞ اﻳﻦ ﭘﮋوﻫﺶ ﻧﺸﺎن داد ﻛﻪ آﻏﺎزﮔﺮﻫﺎی RAPD ﻗﺪرت ﺗﻤﺎﻳﺰ ﺑﺎﻻﻳﻲ داﺷﺘﻪ و در ﺗﻌﻴﻴﻦ ﻣﻴﺰان ﺗﻨﻮع ژﻧﺘﻴﻜﻲ و ﮔﺮوهﺑﻨﺪی ﻻﻳﻦﻫﺎ از ﻛﺎراﻳﻲ ﺑﺎﻻﻳﻲ ﺑﺮﺧﻮر دار ﺑﻮدﻧﺪ.
ﺑﺮای آﻏﺎزﮔﺮ 19 ﻧﺸﺎن دﻫﻨﺪه ﻛﺎراﻳﻲ ﺑﺎﻻی اﻳﻦ آﻏﺎزﮔﺮ در ﺗﻤﺎﻳﺰ ﻻﻳﻦﻫﺎی ﮔﻨﺪم دوروم ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻣﻲﺑﺎﺷﺪ. ﺗﺠﺰﻳﻪ ﺧﻮﺷﻪای ﺑﻪ روش UPGMA و ﺑﺎ اﺳﺘﻔﺎده از ﺿﺮﻳﺐ ﺗﺸﺎﺑﻪ داﻳﺲ اﻧﺠﺎم ﺷﺪ. ﻻﻳﻦﻫﺎی ﻣﻮرد ﺑﺮرﺳﻲ در 3ﺧﻮﺷﻪ ﺟﺪاﮔﺎﻧﻪ ﻃﺒﻘﻪﺑﻨﺪی ﮔﺮدﻳﺪ. ﻫﻢﭼﻨﻴﻦ،ﻧﺘﺎﻳﺞ اﻳﻦ ﭘﮋوﻫﺶ ﻧﺸﺎن داد ﻛﻪ آﻏﺎزﮔﺮﻫﺎی RAPD ﻗﺪرت ﺗﻤﺎﻳﺰ ﺑﺎﻻﻳﻲ داﺷﺘﻪ و در ﺗﻌﻴﻴﻦ ﻣﻴﺰان ﺗﻨﻮع ژﻧﺘﻴﻜﻲ و ﮔﺮوهﺑﻨﺪی ﻻﻳﻦﻫﺎ از ﻛﺎراﻳﻲ ﺑﺎﻻﻳﻲ ﺑﺮﺧﻮر دار ﺑﻮدﻧﺪ.
کلیدواژه ها
Title
Assessment genetic diversity of durum wheat lines using RAPD marker
Authors
Abstract
bstract
The first step for breeding programs is having enough information about genetic diversity among available genotypes. So, in this study the genetic diversity of 20 durum wheat lines was evaluated using RAPD marker. Fourteen primers from 50 used RAPD primers produced 108 polymorphic bands. The polymorphism percentage for all primers was 100%. The lowest number of bands belonged to primer no 25 and the highest number of bands belonged to primer no 30 and 33. The high amount of effective number of alleles, Nei's genetic diversity, Shannon's information index and PIC for primer no 19 indicated high performance of this primer in identifying durum wheat lines. Cluster analysis was performed using UPGMA method and Dice's similarity coefficient. The studied lines were classified in 3 separate clusters. Also, the result of this study showed that the RAPD primers were highly informative and efficient in detecting genetic variability and grouping of lines.
The first step for breeding programs is having enough information about genetic diversity among available genotypes. So, in this study the genetic diversity of 20 durum wheat lines was evaluated using RAPD marker. Fourteen primers from 50 used RAPD primers produced 108 polymorphic bands. The polymorphism percentage for all primers was 100%. The lowest number of bands belonged to primer no 25 and the highest number of bands belonged to primer no 30 and 33. The high amount of effective number of alleles, Nei's genetic diversity, Shannon's information index and PIC for primer no 19 indicated high performance of this primer in identifying durum wheat lines. Cluster analysis was performed using UPGMA method and Dice's similarity coefficient. The studied lines were classified in 3 separate clusters. Also, the result of this study showed that the RAPD primers were highly informative and efficient in detecting genetic variability and grouping of lines.
Keywords
genetic diversity, durum wheat, RAPD marker