تأثیر تنظیم کننده های رشد گیاهی بر کالوسزایی در خرما، رقم استعمران

Date palm (Phoenix dactylifera L.) is a strategic product in terms of nutrition security, job creation and economical value. Micropropagation techniques have successfully method to prevail of vegetative propagation problems. The present study was micropropagation of Date palm through callogenesis on shoot tip explants. All explants were collected from nursery plant, Ramin University of Agriculture and Natural Resources, Khozestan. This research, were conducted in factorial experiments model with completely randomized design, with 4 replicates. The explants, were cultured on MS medium (Murashige and Skoog (1962), supplemented with plant growth regulators including of Thidiazuron (TDZ) and 2.4-dichlorophenoxyacetic acid (2,4.D), at 3 concentrations (0, 5 and 10 mgl-1). The highest levels of callogenesity were observed in 0 2,4.D with 10 mgl-1 TDZ; 5 mgl-1 TDZ and 10 mgl-1 2,4.D; also, in 10 mgl-1 of 2,4.D and TDZ. free MS medium was used to induced embriogenic callus. Maximum callus differentiated into somatic embryos were observed in treatment 10 2,4.D fellow with 10 mgl-1 TDZ. The results showed that is micropropagation possibility in CV. Stameran with used of plant growth regulators (2,4.D and TDZ).