ﺑﺮرﺳﻲ اﺛﺮات ﻫﻮرﻣﻮن 2,4-D ﺑﺮ ﻛﺎﻟﻮس زاﻳﻲ ﮔﻴﺎه ﺳﺮو ﻛﻮﻫﻲ

Genus Juniper (Juniperu sp) from Cupressaceae family is one of the most spread plant species of conifers and is native to temperate regions of Eurasia and North America (Earle, 2006). Plants from Genus Juniperus are very important because of their biological and pharmacological properties. It is grown very well in Iran and contains plenty of oil. Like cone material (fruit), Juniper is Common for having Terpinene-4-ol and is using for centuries as a diuretic. In present study effects of using different concentrations of 2,4-D on callus induction and callus diameter were considered. Culture medium that used for this work was WPM and the purpose of this study was finding a method for propagating this plant. Hormone 2,4-D were used on 5 levels based on a completely randomized design with three replications. Analysis of variance showed that the concentration of 1 mg l-1 2,4-D has the greatest effect on callus induction rate and callus diameter.